研究業績

過去25年間の研究業績(1983-2008)



2007年

原著論文
  1. Honma N, Takubo K, Akiyama F, Kasumi F, Sawabe M, Arai T, Hosoi T, Yoshimura N, Harada N, Sakamoto G.
    Expression of oestrogen receptor-beta in apocrine carcinomas of the breast.
    Histopathology 50(4):425-433, 2007
    [PubMed]


2006年

原著論文
  1. Honma N, Takubo K, Sawabe M, Arai T, Akiyama F, Sakamoto G, Utsumi T, Yoshimura N, Harada N.
    Estrogen-metabolizing enzyme in breast cancers from women over the age of 80 years.
    J Clin Endocrinol Metab. 91(2):607-613,2006
    [PubMed]


2005年

原著論文
  1. Kristensen VN, Sorlie T, Geisler J, Yoshimura N, Linegjaerde OC, Glad I, Friegessi A, Harada N, Lonning PE, Borresen-Dale AL.
    Effects of anastrozole on the intratumoral gene expression in locally advanced breast cancer.
    J Steroid Biochem Mol Biol. 95(1-5):105-111,2005
    [PubMed]
  2. Kristensen VN, Sorlie T, Geisler J, Langerod A, Yoshimura N, Karesen R, Harada N, Lonning PE, Borresen-Dale AL.
    Gene expression profiling of breast cancer in relation to estrogen receptor status and estrogen-metabolizing enzymes: clinical implications.
    Clin Cancer Res. 15:878S-883S,2005 
    [PubMed]
  3. Yamaguchi Y, Takei H, Suemasu K, Kobayashi Y, Kurosumi M, Harada N, Hayashi S.
    Tumor-stromal interaction through the estrogen-signaling pathway in human breast cancer.
    Cancer Res. 65(11):4653-4662, 2005 
    [PubMed]
  4. Harada N, Honda S.
    Analysis of spatiotemporal regulation of aromatase in brain using transgenic mice.
    J Steroid Biochem Mol Biol. 95(1-5):49-55,2005 
    [PubMed]
  5. Utsumi T, Leese MP, Chander SK, Gaukroger K, Purohit A, Newman SP, Potter BV, Reed MJ.
    The effects of 2-methoxyoestrogen sulphamates on the in vitro and in vivo proliferation of breast cancer cells.
    J Steroid Biochem Mol Biol. 94(1-3):219-27,2005 
    [PubMed]
  6. Irahara N, Miyoshi Y, Taguchi T, Tamaki Y, Noguchi S, Harada N.
    Possible involvemet of aromatase over expression induced by cyclo-oxygenase-2 in the pathogenesis of idiopathic gynecomastia.
    Endocr. Res. 31(3):219-27,2005
    [PubMed]


2004年

原著論文
  1. Yoshimura N, Harada N, Bukholm I, Karesen R, Borresen-Dale AL, Kristensen VN.
    Intratumoural mRNA expression of genes from the oestradiol metabolic pathway and clinical and histopathological parameters of breast cancer.
    Breast Cancer Res. 6(2):R46-55,2004
    [PubMed]


2003年


原著論文

  1. Honma, N., Sakamoto, G., Akiyama, F., Rsaki, Y., Sawabe, M., Arai, T., Hosoi, T., Harada, N., Younes, M., & Takubo, K.
    Breast carcinoma in women over the age of 85: distinct histological pattern and androgen, oestrogen, and progesterone receptor status.
    Histology: 42, 120-127, 2003
    [PubMed]
  2. Aste, N., Honda, S., & Harada, N.
    Forebrain fos responses to reproductively related chemosensory cues in aromatase knockout mice.
    Brain Res. Bull. 60, 191-200, 2003
    [PubMed]
  3. Evrard, H.C., Willems, E., Harada, N., & Balthazart, J.
    Specific innervation of aromatase neurons by substance P fibers in the dorsal horn of the spinal cord in quail.
    J. Comp. Neurol. 465, 309-318, 2003
    [PubMed]
  4. Danilovich, N., Harada, N., Sairam, M.R., & Maysinger, D.
    Age-related neurodegenerative changes in the central nervous system of estrogen-deficient follitropin receptor knockout mice.
    Exp. Neurol. 183, 559-572, 2003
    [PubMed]
  5. Harada, N., Yoshimura, N., & Honda, S.,
    Unique regulation of expression of human aromatase in the placenta.
    J. Steroid Biochem. Mol. Biol. 86, 327-334, 2003
    [PubMed]
  6. Matsumoto, T., Honda, S., & Harada, N.
    Neurological effects of aromatase deficiency in the human.
    J. Steroid Biochem. Mol. Biol. 86, 357-365, 2003
    [PubMed]
  7. Matsumoto, T., Honda, S., & Harada, N.
    Alteration in sex-specific behaviors in male mice lacking the aromatase gene.
    Neuroendocrinology: 77, 416-424, 2003
    [PubMed]

著書

和文(総説その他)

  1. 原田信広
    アロマターゼの分子生物学的特性-乳癌における遺伝子発現
    乳癌の抗アロマターゼ療法(医科学出版社)、86-99、2003

学会発表

国内

  1. 性行動の発達と社会適応 原田信広 日本動物学会第74回大会、2003
  2. 女性ホルモンの神経保護作用を含む多様な神経生理作用 原田信広 日本生物物理学会、第41回年会、2003
  3. アロマターゼの脳特異的プロモーターに結合するタンパク質の解析 本田伸一郎、原田信広 第11回日本ステロイドホルモン学会、2003




2002年


原著論文

  1. Yamada, H., Gohyama, N., Honda, S., Hara, T., Harada, N., & Oguri, K.
    Estrogen-dependent regulation of the expression of hepatic Cyp2b and 3a isoforms: Assessment using aromatas-deficient mice.
    Toxicol. Appl. Pharmacol. 180, 1-10, 2002
    [PubMed]
  2. Plumari, L., Viglietti-Panzica, C., Allieri, F., Honda, S., Harada, N., Absil, P., Balthazart, J., & Panzica, G. C.
    Changes in the arginine-vasopressin immunoreactive systems in male mice lacking a functional aromatase gene.
    J. Neuroendocrinol. 14, 1-13, 2002
    [PubMed]
  3. Zhang, Z., Yamashita, H., Toyama, T., Hara, Y., Omoto, Y., Sugiura, H., Kobayashi, S., Harada, N., & Iwase, H.
    Semi-quantitative immunohistochemical analysis of aromatase expression in ductal carcinoma in situ of the breast.
    Breast Cancer Res. Treat. 74, 47-53, 2002
    [PubMed]
  4. Bakker, J., Honda, S., Harada, N., & Balthazart, J.
    Sexual partner preference requires a functional aromatase (Cyp19) gene in male mice.
    Horm. Behav. 42, 158-171, 2002
    [PubMed]
  5. Kato, S., Mano, T., Kobayashi, T., Yamazaki, N., Himeno, Y., Yamamoto, K., Itoh, M., Harada, N., & Nagasaka, A.
    A calcium-deficient diet caused decreased bone mineral density and secondary elevation of estrogen in aged male rats-effect of menatetrenone and elcatonin.
    Metabolism: 51, 1230-4, 2002
    [PubMed]
  6. Bakker, J., Honda, S., Harada, N., & Balthazart, J.
    The aromatase knock-out mouse provides new evidence that estradiol is required during development in the female for the expression of Sociosexual behaviors in adulthood.
    J. Neurosci. 22, 9104-9112, 2002
    [PubMed]
  7. Krohmer, R. W., Bieganski, G. J., Baleckaitis, D. D., Harada, N., Balthazart, J.
    Distribution of aromatase immunoreactivity in the forebrain of the red-sided garter snake.
    J. Chem. Neuroanat. 23, 59-71, 2002
    [PubMed]

著書

和文(総説その他)

  1. 全層角膜移植術後のCandida parapsilosisによる角膜真菌症の1例-原因真菌の薬剤感受性及びItraconazoleの内服を中心とした治療経過について-(山本直樹、原田信広、丸野内棣、日比野勤、早川敏、糸永興一郎、桐渕恵嗣、馬嶋清如)あたらしい眼科、19(3)、367-369、2002
  2. 遺伝子破壊及び改変マウスを用いたアロマターゼ発現調節機構の解析(原田信広、松本高広、山内兄人、本田伸一郎)性差医学、8、68-75、2002
  3. 性の分化とその異常:非副腎性女性半陰陽(原田信広)新女性医学体系、17、222-228、2002

学会発表

国際

  1. Estradiol is required during development for the expression of odor preferences in male mice. (Bakker, J., Honda, S., Harada, N., Balthazart. J.) 32nd Annual Meeting of Society for Neuroscience, Orlando, Florida, U.S.A., 2002
  2. Aromatase activity in the brain of wild-type and ArKO mice. (Baillien, M., Bakker, J., Honda, S., Harada, N., Balthazart, J.) 32nd Annual Meeting of Society for Neuroscience, Orlando, Florida, U.S.A., 2002
  3. Unique regulation of expression of human aromatase in the placenta. (Harada, N., Yoshimura, N., Honda, S.) 6th International Aromatase Conference, Kyoto, 2002
  4. Neurological effects of aromatase deficiency in the mouse. (Honda, S., Matsumoto, T., Harada, N.) 6th International Aromatase Conference, Kyoto, 2002
  5. Role of estrogen in the regulation of hepatic Cyp2b and 3a forms: assessment using aromatase-deficient mice. (Yamada, H., Gohyama, N., Honda, S., Hara, T., Harada, N., Oguri, K.) 6th International Aromatase Conference, Kyoto, 2002
  6. Expression of genes involved in the estradiol metabolic and signalling pathways in tumours from breast cancer patients treated with neoadjuvant aromatase inhibitor anastrozole (ARIMIDEX). (Kristensen, V., Geisler, J., Yoshimura, N., Harada, N., Lonning, P.E., Borresen-Dale, A.-L.) 6th International Aromatase Conference, Kyoto, 2002
  7. Analysis of tmor-stromal interactions in breast cancer b visualization of estrogen signaling pathway. (Yamaguchi, Y., Omoto, Y., Takei, H., Suemasu, K., Harada, N., Hayashi, S.) 6th International Aromatase Conference, Kyoto, 2002
  8. The intracrinological estrogen metablism in breast cancer cell lines. (Sakamoto ,H., Utsumi, T., Honda, S., Yoshimura, N., Sasaki, E., Harada, N.) 6th International Aromatase Conference, Kyoto, 2002
  9. New approaches to the understanding of tamoxefen action and resistance. (Berstein, L.M., Zheng, H., Yu, W., Wang, J,-P., Lykkesfeldt, A,E., Dowsett, M., Detre, S., Miller, W.R., Naftolin, F., Harada, N., Shanabrough, M., Santen, R.J.) 11th International Congress on Hormonal Steroids/7th International Congress on Hormones and Cancer, Fukuoka, 2002
  10. Intratumoral mRNA expression of genes from the oestradiol metabolic pathway and clinical and histopathological parameters of breast cancer. (Yoshimura, N., Harada, N., Bukholm, I., Kaaresen, R., Borrensen-Dale, A.-L., Kristensen, V.N.) 11th International Congress on Hormonal Steroids/7th International Congress on Hormones and Cancer, Fukuoka, 2002
  11. Promoter analysis and physiological function of brain aromatase. (Honda, S., Sakamoto, H., Harada, N.) 11th International Congress on Hormonal Steroids/7th International Congress on Hormones and Cancer, Fukuoka, 2002
  12. The anti-proliferative effects of aromatase and steroid sulfatase inhibitors on endometrial cancer cells. (Nishio, E., Yoshimura, N., Sakamoto, H., Udagawa, Y., Harada, N.) 11th International Congress on Hormonal Steroids/7th International Congress on Hormones and Cancer, Fukuoka, 2002
  13. The regulation of in situ estrogen activity in human endometrial carcinoma. (Utsunomiya, H., Suzuki, T., Kaneko, C., Kitamura, T., Harada, N., Ito, K., Yaegashi, N., Okamura, K., Sasano, H.) 11th International Congress on Hormonal Steroids/7th International Congress on Hormones and Cancer, Fukuoka, 2002
  14. Estradiol is required during development for the expression of odor preferences in male mice. (Bakker, J., Honda, S., Harada, N., Balthazart, J.) 32nd Annual Meeting of Society for Neuroscience, Orlando, Florida, U.S.A., 2002
  15. Aromatase activity in the brain of wild-type and ArKO mice. (Baillien, M., Bakker, J., Honda, S., Harada, N., Balthazart, J.) 32nd Annual Meeting of Society for Neuroscience, Orlando, Florida, U.S.A., 2002
  16. Switching mechanism of multiple exons 1 of the human aromatase gene. (Sakamoto, H., Honda, S., Yoshimura, N., Sasaki, E., Harada, N.) 18th International Congress of Clinical Chemistry and Laboratory Medicine, Kyoto, 2002
  17. Changes in the neuronal nitric oxide synthase immunoreactive system in male mice lacking a functional aromatase gene. (Sica, M., Plumari, L., Honda, S., Harada, N., Absil, P., Viglietti-Panzica, C., Balthazart, J., Panzica, G.C.) Society for Behavioral Neuroendocrinology Annual Meeting, Amherst, MA, U.S.A., 2002
  18. New evidence that estradiol is required during development in the female mouse for the expression of socio-sexual behaviors in adulthood. (Bakker, J., Honda, S., Harada, N., Balthazart, J.) Society for Behavioral Neuroendocrinology Annual Meeting, Amherst, MA, U.S.A., 2002




2001年


原著論文

  1. Takeyama, J., Suzuki, T., Inoue, S., Kaneko, C., Nagura, H., Harada, N., & Sasano, H.
    Expression and cellular localization of estrogen receptors a and b in the human fetus.
    J. Clin. Endocrinol. Metab. 86, 2258-2262, 2001
    [PubMed]
  2. Tsubota, T., Taki, S., Nakayama, K., Mason, J. I., Kominami, S., Harada, N., & Kita, I.
    Immunolocalization of steroidogenic enzymes in the corpus luteum and placenta of the Japanese black bear, Ursus thibetanus japonicus, during pregnancy.
    Reproduction; 121, 587-594, 2001
    [PubMed]
  3. Azcoitia I, Sierra, A., Veiga, S., Honda, S., Harada, N., & Garcia-Segura, L. M.
    Brain aromatase is neuroprotective.
    J. Neurobiol. 47, 318-29, 2001
    [PubMed]
  4. Utsunomiya H, Suzuki T, Kaneko C, Takeyama J, Nakamura J, Kimura K, Yoshimura M, Harada N, Ito K, Konno R, Sato S, Okamura K, & Sasano H.
    The analysis of 17b-hydroxysteroid dehydrogenase isozymes in human endometrial hyperplasia and carcinoma.
    J. Clin. Endocrinol. Metab. 86, 3436-3443, 2001
    [PubMed]
  5. Hayashi, T., Ohta, Y., Inagaki, S., & Harada, N.
    Inhibitory action of Oren-gedoku-to extract on enzymatic lipid peroxidation in rat liver microsomes.
    Biol. Pharm. Bull. 24, 1165-1170, 2001
    [PubMed]
  6. Kristensen, VN., Harada, N., Kristensen, T., & Borresen-Dale, AL.
    Genetic polymorphism and variability of steroid hormone metabolism: connection with risk of developing breast neoplasms.
    Voprosy Onkologii: 47, 156-159, 2001
    [PubMed]
  7. Ishinazaka, T., Suzuki, M., Yamamoto, Y., Isono, T., Harada, N., Mason, J.I., Watabe, M., Tsunokawa, M., & Ohtaishi, N.
    Immunohistochemical localization of steroidogenic enzymes in the corpus luteum and the placenta of the ribbon seal (Phoca fasciata) and steller sea lion (Eumetopias jubatus).
    J. Vet. Med. Sci. 63, 955-959, 2001
    [PubMed]
  8. Sano, T., Hirasawa, G., Takeyama, J., Darnel, A.D., Suzuki, T., Moriya, T., Kato, K., Sekine, H., Ohara, S., Shimosegawa, T., Nakamura, J., Yoshihama, M., Harada, N., & Sasano, H.
    17b-Hydroxysteroid dehydrogenase type 2 expression and enzyme activity in the human gastrointestinal tract.
    Clin. Sci. 101, 485-491, 2001
    [PubMed]
  9. Murakami, H., Harada, N., & Sasano, H.
    Aromatase in atherosclerotic lesions of human aorta.
    J. Steroid Biochem. Mol. Biol. 79, 67-74, 2001
    [PubMed]
  10. Harada, N., Matsumoto, T., Yoshimura, N., Sakamoto, H., & Honda, S.
    Analysis of transcriptional regulation of human breast aromatase by in vitro and in vivo studies.
    J. Steroid Biochem. Mol. Biol. 79, 151-156, 2001
    [PubMed]
  11. Honda, S., Matsumoto, T., & Harada, N.
    Characterization and purification of a protein binding to the cis-acting element for brain-specific exon 1 of the mouse aromatase gene.
    J. Steroid Biochem. Mol. Biol. 79, 255-260, 2001
    [PubMed]
  12. Kristensen, V. N., Kure, E. H., Erikstein, B., Harada, N., & Borresen-Dale, A.
    Genetic susceptibility and environmental estrogen-like compounds.
    Mutation Res. 482, 77-82, 2001
    [PubMed]

著書

和文(総説その他)

  1. アトピー白内障の水晶体上皮細胞におけるMajor Basic Protein発現と起因についての検討(山本直樹、原田信広、日比野勤、糸永興一郎、桐渕恵嗣、馬嶋清如)あたらしい眼科、18 (3)、359-362、2001
  2. 活性化エネルギーを用いた患者血清中のミトコンドリアCK (mitCK) の出現検索と mitCK出現血清中のMn-SOD濃度について(山舘周恒、太田好次、長村洋一、原田信広)生物試料分析、24 (5)、371-376、2001
  3. ホルモン依存性腫瘍におけるアロマターゼの役割(原田信広)癌の臨床(Jpn. J. Cancer Clin.)、47(10)、807-813、2001
  4. 雌型性行動中枢制御の性分化におけるアロマターゼの役割(本田伸一郎、松本高広、原田信広)ホルモンと臨床、49、156-160,2001

学会発表

国際

  1. The sexually dimorphic arginine-vasopressine immunoreactive system is deeply affected in male mice lacking a functional aromatase enzyme gene. (Panzica, G.C., Plumari, L., Allieri, F., Viglietti-Panzica, C., Honda, S., Harada, N., Absil, P., & Balthazart, J.) 31st Annual Meeting of Society for Neuroscience, San Diego, CA, U.S.A., 2001
  2. Localization of estrogen-synthase (aromatase) in the rat spinal cord. ( Evrard, H.C., Harada, N., Balthazart, J.) 31st Annual Meeting of Society for Neuroscience, San Diego, CA, U.S.A., 2001
  3. The aromatase gene is required for the expression of odor preferences in male and female mice. (Bakker, J., Honda, S., Harada, N., Balthazart, J.) 31st Annual Meeting of Society for Neuroscience, San Diego, CA, U.S.A., 2001
  4. Role of urine derived pheromonal stimuli in the activation of fos immunoreactivity in the rain of aromatase knockout mice. (Aste, N., Honda, S., Harada, N.) International Meeting Steroids and Nervous System, Torino, Italy, 2001

国内

  1. アロマターゼ遺伝子破壊マウスの性行動解析を通した脳の性分化におけるアロマター ゼ・エストロゲン仮説の検証 原田信広、松本高広、本田伸一郎 第44回日本神経化 学会大会、2001
  2. ホルモン依存性腫瘍におけるアロマターゼの役割 原田信広 第60回日本癌学会総会、2001
  3. アロマターゼの臓器特異的な生理機能と発現調節 本田伸一郎、松本高広、笹野公伸、原田信広 第74回日本生化学会大会、2001




2000年


原著論文

  1. Takeyama, J., Suzuki, T., Hirasawa, G., Muramatsu, Y., Nagura, H., Iimura, K., Nakamura, J., Kimura, K., Yoshihama, M., Harada, N., Andersson, S., & Sasano, H.
    17b-Hydroxysteroid dehydrogenase type 1 and 2 expression in the human fetus.
    J. Clin. Endocrinol. Metab. 85, 410-416, 2000
    [PubMed]
  2. Utsumi, T., Yoshimura, N., Maruta, M., Mizoguchi, Y., & Harada, N.
    Correlation of cyclin D1 mRNA levels with clinicopathological parameters and clinical outcome in human breast carcinoma.
    Int. J. Cancer; 89, 39-43, 2000
    [PubMed]
  3. Kristensen, V.N., Harada, N., Yoshimura, N., Haraldsen, E., Lonning, P.E., Erikstein, B., Karesen, R., M., Kristensen, T., & Borresen-Dale, A-L.
    Genetic variations of Cyp19 (aromatase) and breast cancer risk.
    Oncogene; 19,1329-1333, 2000
    [PubMed]
  4. Utsumi, T., Yoshimura, N., Takeuchi, S., Maruta, M., Maeda, K., & Harada, N.
    Elevated steroid sulfatase expression in breast cancers.
    J. Steroid Biochem. Mol. Biol. 73, 141-145, 2000
    [PubMed]
  5. Ohta, Y., Kongo, M., Sasaki, E., Ishiguro, I., & Harada, N.
    Protective effect of melatonin against a-naphthylisothiocyanate-induced liver injury in rats.
    J. Pineal Res. 29, 15-23, 2000
    [PubMed]
  6. Evrard, H., Baillien, M., Foidart, A., Absil, P., Harada, N., & Balthazart, J.
    Localization and controls of aromatase in the quail spinal cord.
    J. Comp. Neurol. 423, 552-564, 2000
    [PubMed]
  7. Balthazart, J., Tlemcani, O., Harada, N., & Baillien, M.
    Ontogeny of aromatase and tyrosine hydroxylase activity and of aromatase-immunoreactive cells in the preoptic area of male and female japanese quail.
    J. Neuroendocrinol. 12, 853-866, 2000
    [PubMed]
  8. Watanabe, T., Yasuda, T., Noda, H., Wada, K., Kazukawa, I., Someya, T., Minamitani, K., Minagawa, M., Wataki, K., Matsunaga, T., Ohnuma, N., Kohno, Y., & Harada, N.
    Estrogen secreting adrenal adenocarcinoma in an 18-month-old boy: Aromatase activity, protein expression , mRNA and utilization of gonadal type promoter.
    Endocrine J. 47, 723-730, 2000
    [PubMed]
  9. Utsunomiya, H., Suzuki, T., Harada, N., Ito, K., Matsuzaki, S., Konno, R., Sato, S., Yajima, A., & Sasano, H.
    Analysis of estrogen receptor a and b in endometrial carcinomas: Crrelation with ERb and clinicopathologic findings in 45 cases.
    Int. J. Gynecol. Pathol. 19, 335-341, 2000.
    [PubMed]

著書

和文(総説その他)

  1. Pseudallesheria sp. が分離された角膜真菌症の一例 -症例報告と原因真菌の薬剤感受性について- (山本直樹、馬嶋清如、井上孝、神戸俊夫、日比野勤、原田信広、堀口正之、馬嶋慶直)臨床と微生物、27 (1)、103-108、2000
  2. アロマターゼの分子生物学とその異常(原田信広)現代医療、32 (7)、1677-1682、2000

学会発表

国際

  1. Aromatase expression in human aorta. (Sasano, H., Murakami, H., & Harada, N.) 5th International Aromatase Conference, Port Douglas, Australia, 2000
  2. ranscriptional regulation and alternative exons 1 swithcing of aromatase in breast cancer tissues. (Harada, N., Sakamoto, H., & Honda, S.) 5th International Aromatase Conference, Port Douglas, Australia, 2000
  3. Regulation of aromatase gene expression by the brain-specific promoter.(Honda, S., Matsumoto, T., & Harada, N.) 5th International Aromatase Conference, Port Douglas, Australia, 2000
  4. Expression of genes from the estradiol metabolic and signalling pathways in tumours of breast cancer patients treated with neoadjuvant aromatase inhibitor anastrozole (Arimidex). (Kristensen, V.N., Geisler, J., Yoshimura, N., Harada, N., Lonning, P.E., & Borresen-Dale, A.-L.) 5th International Aromatase Conference, Port Douglas, Australia, 2000
  5. Expression of cyp19 (aromatase) mRNA, and clinical and histopathological parameters of breast cancers. (Yoshimura, N., Kristensen, V.N., Bukholm, I., Karesen, R., Harada, N., & Borrensen-Dale, A.-L.) 5th International Aromatase Conference, Port Douglas, Australia, 2000




1999年


原著論文

  1. Diano, S., Horvath, T.L., Register, T., Adams, M., Harada, N., & Naftolin, F.
    Aromatase and estrogen receptor immunoreactivity in the coronary arteries of monkeys and human subjects.
    Menopause; 6, 21-28, 1999
    [PubMed]
  2. Utsumi, T., Yoshimura, N., Takeuchi, S., Ando, J., Maruta, M., Maeda, K., & Harada, N.
    Steroid sulfatase expression is an independent predictor of reccurrence in human breast cancer.
    Cancer Res. 59, 377-381, 1999
    [PubMed]
  3. Harada, N., Honda, S., & Hatano, O.
    Aromatase inhibitors and enzyme stability.
    Endocrine-Related Cancer; 6, 211-218, 1999
    [PubMed]
  4. Honda, S., Harada, N., Abe-Dohmae, S., & Takagi, Y.
    Identification of cis-acting elements in the proximal promoter region for the brain specific exon 1 of the mouse aromatase gene.
    Mol. Brain Res. 66, 122-132, 1999
    [PubMed]
  5. Sasano, H., Murakami, H., Shizawa, S., Satomi, S., Nagura, H., & Harada, N.
    Aromatase and sex steroid receptors in human vena cava.
    Endocr. J. 46, 233-242, 1999
    [PubMed]
  6. Harada, N., Sasano, H., Murakami, H., Ohkuma, T., Nagura, H., & Takagi, Y.
    Localized expression of aromatase in human vascular tissues.
    Circ. Res. 84, 1285-1291, 1999
    [PubMed]
  7. Kongo, M., Ohta, Y., Nishida, K., Harada, N., Ishiguro, I.
    An association between lipid peroxidation and a-naphthylisothiocyanate-induced liver injury in rats.
    Toxicology Lett. 105, 103-110, 1999
    [PubMed]
  8. Utsumi, T., Yoshimura, N., Maruta, M., Takeuchi, S., Ando, J., Maeda, K., & Harada, N.
    Significance of steroid sulfatase expression in human breast cancer.
    Breast Cancer; 6, 298-300, 1999
    [PubMed]
  9. Harada, N.
    Aromatase and intracrinology of estrogen in hormone-dependent tumors.
    Oncology; 57, 7-15, 1999
    [PubMed]
  10. Yamada, K., Ogawa, H., Honda, S., Harada, N., & Okazaki, T.
    A GCM motif protein is involved in placenta-specific expression of human aromatase gene.
    J. Biol. Chem. 274, 32279-32286, 1999
    [PubMed]
  11. Ohta, Y., Kongo, M., Sasaki, E., & Harada, N.
    Change in hepatic antioxidant defense system with liver injury development in rats with a single naphthylisothiocyanate intoxication.
    Toxicology; 139, 265-275, 1999
    [PubMed]
  12. Delado惣, J., Fl歡k, C., Bex, M., Yoshimura, N., Harada, N., & Mullis, P.E.
    Aromatase deficiency caused by a novel P450arom gene mutation: Impact of absent estrogen production on serum gonadotropin concentration in a boy.
    J. Clin. Endocrinol. Metab. 84, 4050-4054, 1999
    [PubMed]
  13. Mano, T., Mori, K., Sawai, Y., Oda, N., Tugawa, T., Nakano, I., Watanabe, F., Hamada, M., Nakai, A., Yamamoto, H., Harada, N., Nagasaka, A., & Itoh, M.
    Accelerated conversion of dehydroepiandrosterone sulfate to estrogen in a patient with Crow-Fukase syndrome and diabetes mellitus.
    Endocr. Res. 25, 371-380, 1999
    [PubMed]

著書

欧文(分担執筆共著)

  1. Histological lesions in aromatase-deficiency mice. (Harada, N., Honda, S., Hirasawa, G., Hatano, O., and Sasano, H. ) Molecular Steroidgenesis [M. Okamoto, Y. Ishimura, H. Nawata 編] 133-136, Universal Academic Press, Inc., 1999
  2. Aromatase expression and its regulation in human bone, aorta and brain. (Sasano, H., Murakami, H., Suzuki, T., Takahashi, K., and Harada, N.) Molecular Steroidgenesis [M. Okamoto, Y. Ishimura, H. Nawata 編] 143-136, Universal Academic Press, Inc., 1999
  3. Disruption of sexual behavior in male aromatase-deficient mice. (Honda, S., Harada, N., Ito, S., Takagi, Y., and Maeda, S. ) Molecular Steroidgenesis [M. Okamoto, Y. Ishimura, H. Nawata 編] 151-154, Universal Academic Press, Inc., 1999

学会発表

国際

  1. Expression of 17b-hydroxysteroid dehydrogenase (17b-HSD) type 1 ans type 2 in human fetal tissues. (Takayama, J., Suzuki, T., Iinuma, K., Harada, N., Anderson, S., & Sasano, H. ) International Symposium on Molecular Steroidgenesis, Nara, Japan, 1999
  2. Ontogeny of aromatase sex differences in the quail preoptic area. (Foidart, A., Baillien, M., Tlemcani, O., Harada, N., Balthazart, J.) 29th Annual Meeting of Society for Neuroscience, Miami Beach, Florida, U.S.A., 1999
  3. Localization of estrogen-synthase (aromatase) in the rat spinal cord. (Evrard, H., Harada, N., Balthazart, J.) 29th Annual Meeting of Society for Neuroscience, Miami Beach, Florida, U.S.A., 1999
  4. Genetic polymorphism of CYP19 (aromatase), breast cancer risk and clinical and histopathological parameters. (Nedelcheva, V.K., Harada, N., Haraldson, E., Yoshimura, N., Lonning, P.E., Erikstein, B., Karesen, R., Kristensen, T., & Borrensen-Dale, A-L. ) International Symposium on Molecular Steroidgenesis, Nara, Japan, 1999
  5. Histological lesions in aromatase-deficiency mice. (Harada, N., Honda, S., Hirasawa, G., Hatano, O., & Sasano, H. ) International Symposium on Molecular Steroidgenesis, Nara, Japan, 1999
  6. Aromatase expression and its regulation in human bone, aorta and brain. (Sasano, H., Murakami, H., Suzuki, T., Takahashi, K., & Harada, N.) International Symposium on Molecular Steroidgenesis, Nara, Japan, 1999
  7. Disruption of sexual behavior in male aromatase-deficient mice. (Honda, S., Harada, N., Ito, S., Takagi, Y., & Maeda, S. ) International Symposium on Molecular Steroidgenesis, Nara, Japan, 1999

国内

  1. エストロゲン合成酵素の発現制御系とその生理機能の比較内分泌学 原田信広 第24回比較内分泌学会大会、1999
  2. 乳癌の治療においてエストロゲン生成を律速、制御する酵素群の発現レベルの重要性  原田信広、吉村憲子、内海俊明 第37回日本癌治療学会総会、1999




1998年


原著論文

  1. Harada, N. & Hatano, O.
    Inhibitors of aromatase prevent degaradation of the enzyme in cultured human tumour cells.
    Br. J. Cancer; 77, 567-572, 1998
    [PubMed]
  2. Harada, N., Ota, H., Yoshimura, N., Katsuyama, T., & Takagi, Y.
    Localized aberrant expression of cytochrome P450 aromatase in primary and metastatic malignant tumors of human liver.
    J. Clin. Endocrinol. Metab. 83, 697-702, 1998
    [PubMed]
  3. Sasano, H., Takahashi, K., Sato, F., Nagura, H., & Harada, N.
    Aromatase in human central nervous system.
    Clin. Endocrinol. 48, 325-329, 1998
    [PubMed]
  4. Balthazart, J., Foidart, A., Baillien, M., Harada, N., & Ball, G.F.
    Anatomical relationships between aromatase and tyrosine hydroxylase in the quail brain: Double-label immunocytochemical studies.
    J. Comp. Neurol. 391, 214-226, 1998
    [PubMed]
  5. Aste, N., Panzica, G.C., Viglietti-Panzica, C., Harada, N., & Balthazart, J.
    Distribution and effects of testosterone on aromatase mRNA in the quail forebrain: a non-radioactive in situ hybridization study.
    J. Chem. Neuroanat. 14, 103-115, 1998
    [PubMed]
  6. Sasano, H. Suzuki, T., & Harada N.
    From endocrinology to incrinology.
    Endocrine Pathology ; 9, 9-20, 1998
    [PubMed]
  7. Sasano, H & Harada, N.
    Intratumoral aromatase in human breast, endometrial and ovarian malignancies.
    Endocrine Reviews; 19, 593-607, 1998
    [PubMed]
  8. Foidart, A., Silverin, B., Baillien, M., Harada, N., & Balthazart, J.
    Neuroanatomical distribution and variations across the reproductive cycle of aromatase activity and aromatase-immunoreactive cells in the pied flycatcher (Ficedula hypoleuca).
    Horm. Behav. 33, 180-196, 1998
    [PubMed]
  9. Nomura, M., Kawabe, K., Matsushita, S., Oka, S., Hatano, O., Harada, N., Nawata, H., & Morohashi, K.
    Adrenocortical and gonadal expression of the mammalian Fts-F1 gene encoding Ad4BP/SF-1 is independent of pituitary control.
    J. Biochem. 124, 217-224, 1998
    [PubMed]
  10. Harada, N., & Honda, S.
    Molecular analysis of aberrant expression of aromatase in breast cancer tissue.
    Breast Cancer Res. Treat. 49, 15-21, 1998
    [PubMed]
  11. Santen, R.J., Martel, J., Hoagland, M., Naftolin, F., Roa, L., Harada, N., Hafer, L., Zaino, R., Pauley, R., & Santner, S.
    Demonstration of aromatase activity and its regulation in breast tumor and benign breast fibroblasts.
    Breast Cancer Res. Treat. 49, 93-99, 1998
    [PubMed]
  12. Mor, G., Yue, W., Santen, R.J., Gutierrez, L., Eliza, M., Berstein, L.M., Harada, N., Wang, J., Lysiak, J., Diano, S., & Naftolin, F.
    Macrophages, estrogen and the microemvironment of breast cancer.
    J. Steroid Biochem. Mol. Biol. 67, 403-411, 1998
    [PubMed]
  13. Honda, S., Harada, N., Ito, S., Takagi, Y., & Maeda, S.
    Disruption of sexual behavior in male aromatase-deficient mice lacking exons 1 and 2 of the cyp19 gene.
    Biochem. Biophys. Res. Commun. 252, 445-449, 1998
    [PubMed]

著書

和文(総説その他)

  1. 動物のステロイドホルモン合成とP450 (岡本光弘、原田信広、園部治之)化学と生物、36 (9), 612-618, 1998
  2. 幼少期より多嚢胞性卵巣や骨代謝異常を呈するアロマターゼ完全欠損症の1例(原田信広)ホルモンと臨床、46、215-219、1998
  3. アロマターゼ遺伝子 (原田信広)小児内科、30 (8)、1038-1041、1998
  4. アロマターゼ・エストロゲン系の持つ新たな生理・薬理学的意義 (原田信広)日薬理誌(Folia Pharmacol. Jpn.)112、51-58、1998

学会発表

国際

  1. Aromatase inhibitors and enzyme stability. (Harada, N., Honda, S., & Hatano, O.) International Symposium of Aromatase and Its Inhibitors. Prague, Czech Republic, 1998




1997年


原著論文

  1. Mullis, P.E., Yoshimura, N., Kuhlmann, B., Lippuner, K., Jaeger, P., & Harada, N.
    Aromatase deficiency in a girl compound heterozygote for two new point mutations in the P450arom gene: impact of estroges on hypergonadotropic hypogonadism, multicystic ovaries, body composition and bone densitometry in childhood.
    J. Clin. Endocrinol. Metab. 82, 1739-1745, 1997
    [PubMed]
  2. Abe-Dohmae, S., Harada, N., & Takagi, Y.
    Autonomous expression of aromatase during development of mouse brain is modulated by neurotransmitters.
    J. Steroid Biochem. Mol. Biol. 61, 299-306, 1997
    [PubMed]
  3. Harada, N.
    Aberrant expression of aromatase in breast cancer tissues.
    J. Steroid Biochem. Mol. Biol. 61, 175-184, 1997
    [PubMed]
  4. Hiramatsu, M., Maehara, I., Ozaki, M., Harada, N., Orikasa, S., & Sasano, H.
    Aromatase in hyperplasia and carcinoma of the human prostate.
    The Prostate; 31, 118-124, 1997
    [PubMed]
  5. Sasano, H., Uzuki, M., Sawai, T., Nagura, H., Matsunaga, G., Kashimoto, O., & Harada, N.
    Aromatase in human bone tissue.
    J. Bone Miner. Res. 12, 1416-1423, 1997
    [PubMed]
  6. Watanabe, J., Harada, N., Suemasu, K., Higashi, Y., Gotoh, O., & Kawajiri, K.
    Arginine-cysteine polymorphism at codon 264 of the human CYP19 gene does not affect aromatase activity.
    Pharmacogenetics; 7, 419-424, 1997
    [PubMed]

著書

和文(総説その他)

  1. アロマターゼ遺伝子の発現調節と発現異常 (原田信広)The Bone, 11(1), 47-54, 1997
  2. PCRを用いた定量的実験 (原田信広)PCR実験ノート(羊土社)60-72, 1997

学会発表

国際

  1. Immunohistochemical localization of steroidogenic enzymes within the porcine follicle. (Shores, E., Picton, H.M., Harada, N., Hunter, M.G.) International Ovarian workshop, Bristol, England, 1997
  2. Comparison of the distribution of steroidogenic enzymes and hormone production during follicular development in the pig. (Shores, E., Picton, H.M., Harada, N., hunter, M.G.) Annual Meeting of Society for the Study of Fertility, York, England, 1997
  3. Immunolocalization of steroidogenic enzymes in the corpus luteum and the placenta of Japanese black bears, Ursus Thibetanus Japonicus during pregnancy. (Tsubota, T., Taki, S., Nakayama, K., Mason, J.I., Kominami, S., Harada, N., Kita, I.) Annual Meeting of Society for the Study of Reproduction, Portlamd, OR, U.S.A., 1997
  4. Additional markers for the sexually dimorphic necleus of the quail preoptic area: aromatase mRNA as detected by in situ hybridization and projections to the central gray identified by retrograde tracing. (Riters, L.V., Aste, N., Panzica, G.C., Harada, N., & Balthazart, J.) 27th Annual Meeting of Society for Neuroscience, New Orleans, LA, U.S.A., 1997
  5. Anatomical specificity in the colocalization of estrogen receptors and aromatase in the quail brain: effects of castration and testosterone replacement. (Foidart, A., Houbart, M., Harada, N., & Balthazart, J.) Annual Meeting of Belgium Society for Neuroscience, Brussels, Belgium, 1997
  6. Neuroanatomical distribution of aromatase-immunoreactive cells in the zebra finch brain and the definition of preoptic nuclei. (Absil, P., Ball, G.F., Harada, N., & Balthazart, J.) Annual Meeting of Belgium Society for Neuroscience, Brussels, Belgium, 1997
  7. The testosterone-sensitive and sexually dimorphic aromatase-immunoreactive cells in the quail preoptic area: a tridimensional study. (Tlemcani, O., Harada, N., & Balthazart, J.) Annual Meeting of Belgium Society for Neuroscience, Brussels, Belgium, 1997
  8. Molecular analysis of aberrant expression of aromatase in breast cancer tissues. (Harada, N. & Honda, S.) Intratumoral Aromatase Workshop, Winterhur, Switzerland, 1997




1996年


原著論文

  1. Conley, A.J., Corbin, C.J., Hinshelwood, M.M., Liu, Z., Simpson, E.R., Ford, J.J., & Harada, N.
    Functional aromatase expression in porcine adrenal gland and testis.
    Biol. Reprod. 54, 497-505, 1996
    [PubMed]
  2. Abe-Dohmae, Tanaka, R., Takagi, Y., & Harada, N.
    In vitro increase of aromatase mRNA in diencephalic neurons.
    Neuroendocrinology; 63, 46-52, 1996
    [PubMed]
  3. Honda, S., Harada, N., & Takagi, Y.
    The alternative exons 1 of the mouse aromatase cytochrome P-450 gene.
    Biochim. Biophys. Acta ; 1305, 145-150, 1996
    [PubMed]
  4. Balthazart, J., Foidart, A., Absil, P., & Harada, N.
    Effects of testosterone and its metabolites on aromatase-immunoreactive cells in the quail brain: Relationship with the activation of male reproductive behavior.
    J. Steroid Biochem. Molec. Biol. 56, 185-200, 1996
    [PubMed]
  5. Naftolin, F., Horvath, T.L., Jakab, R.L., Leranth, C., Harada, N., & Balthazart, J.
    Aromatase immunoreactivity in axon terminals of the vertebrate brain: An electron microscopic immunocytochemical study on quail, rat, monkey, and human tissues.
    Neuroendocrinology; 63, 149-155, 1996
    [PubMed]
  6. Utsumi, T., Harada, N., Maruta, M., & Takagi, Y.
    Presence of alternatively spliced transcripts of aromatase gene in human breast cancer.
    J. Clin. Endocrinol. Metab; 81, 2344-2349, 1996
    [PubMed]
  7. Sasano, H., Kaga, K., Sato, S., Yajima, A., Nagura, H., & Harada, N.
    Aromatase cytochrome P450 gene expression in endometrial carcinoma: An anlysis of alternative splicing of exons 1 in seven cases.
    Br. J. Cancer ; 74, 1541-1544, 1996
    [PubMed]
  8. Rissman, E.F., Harada, N., & Roselli, C.E.
    Effect of vorozole, an aromatase enzyme inhibitor, on sexual behavior, aromatase activity and neural immunoreactivity.
    J. Neuroendocrinol. 8, 199-210, 1996
    [PubMed]
  9. Araki, H., Tsubota, T., Maeda, N., Harada, N., Kominami, S., Mason, J.I., and Kita, I.
    Intraovarian immunolocalization of steroidgenic enzymes in a Hokkaido brown bear, Ursus arctos yesoensis during the mating season.
    J. Vet. Med. Sci. 58 (8), 787-790, 1996
    [PubMed]
  10. Kaga, K., Sasano, H., Harada, N., Ozaki, M., Sato, S., & Yajima, A.
    Aromatase in human common epithelial ovarian neoplasms.
    Am. J. Pathol. 149, 45-51,1996
    [PubMed]
  11. Isurugi, K., Hasegawa, F., Shibahara, N., Mori, H., Shima, H., Harada, N., Hasegawa, T., Honma, S., Imasaki, K., & Nawata, H.
    Incomplete testicular feminization syndrome: studies on androgen receptor (AR) function, AR geneanalysis, and aromatase activities at puberty and long-term observations of clinical and hormonal features from infancy to puberty
    Endocrine J. 43, 557-564, 1996
    [PubMed]
  12. Abe-Dohmae, S., Takagi, Y., & Harada, N.
    Neurotransmitter-mediated regulation of brain aromatase: protein kinase C- and G-dependent induction.
    J. Neurochem. 67, 2087-2095, 1996
    [PubMed]
  13. Balthazart, J., Absil, P., Houbart, M., Harada, N., Ball, G.F.
    Distribution of Aromatase-immunoreactive cells in the forebrain of zebra finches (Taeniopygia guttata): Implication for the neural action of steroids and nuclear definition in the avian hypothalamus.
    J. Neurobiol. 31, 129-148, 1996
    [PubMed]
  14. Balthazart, J., Tlemcani, O., & Harada, N.
    Localization of testosterone-sensitive and sexually dimorphic aromatase-immunoreactive cells in the quail preoptic area.
    J. Chem. Neuroanatomy; 11, 147-171, 1996
    [PubMed]
  15. Sasano, H., Frost, A.R., Saito R., Harada, N. , Poutanen, M., Vihko, R., Bulun, S.E., Silverberg, S.G., Nagura, H.
    Aromatase and 17beta-hydroxysteroid dehydrogenase type 1 in human breast carcinoma.
    J. Clin. Endocrinol. Metab. 81, 4042-4046, 1996
    [PubMed]
  16. Tsugaya, M., Harada, N., Tozawa, K., Yamada, Y., Hayashi, Y., Tanaka, S., Maruyama, K., & Kohri, K.
    Aromatase mRNA levels in benign prostatic hyperplasia and prostate cancer.
    Int. J. Urol. 3, 292-296, 1996
    [PubMed]

著書

和文(総説その他)

  1. アロマターゼ研究の最近の進歩 (原田信広)、ホルモンと臨床、44 (6), 565-573, 1996
  2. アロマターゼの組織特異的発現調節 (原田信広)、Clinical Calcium, 6 (11), 20-24, 1996

学会発表

国際

  1. Changes in steroidgenic sites during pregnancy in the domestic cat. (Tsubota, T., Araki, H., Mason, J. I., Harada, N., Kita, I.) 29th Annual Meeting of Society for the Study of Reproduction, London, Canada, 1996
  2. A three dimensional study of testosterone-sensitive and sexually dimorphic aromatase-immunoreactive cells in the quail preoptic area. (Balthazart, J., Tlemcani, O., Harada, N.) Italian Journal of Anatomy and Embryology, 101. Suppl. 1, 142-143, 1996
  3. Immunocytochemical localization of aromatase-immunoreactive cells and the difinition of preoptic nuclei in the zebra finch brain. (Absil, P., Ball, G. F., Harada, N., Balthazart, J.)26th Annual Meeting of Society for Neuroscience, Washington, DC., U.S.A., 1996
  4. Effects of castration and testosterone replacement on the colocalization of estrogen receptors and aromatase in the quail brain. (Foidart, A., Houbart, M., Harada, N., Balthazart, J.) 26th Annual Meeting of Society for Neuroscience, Washington, DC., U.S.A., 1996
  5. Control of aromatase mRNA in cultured diencephalic neurons. (Abe-Dohmae, S., Harada, N., Takagi, Y.) 4th International Aromatase Conference, Tahoe City, CA, U.S.A., 1996
  6. Steroid control and sexual differentiation of brain aromatase. (Balthazart, J., Foidart, A., Tle溝ni, O., Harada, N.) 4th International Aromatase Conference, Tahoe City, CA, U.S.A., 1996
  7. Aromatase in human central nervous system. (Sasano, H., Yakahashi, K., Nagura, H., Harada, N.) 4th International Aromatase Conference, Tahoe City, CA, U.S.A., 1996
  8. Analysis of the proximal promoter region of brain-specific aromatase exon 1.(Honda, S., Harada, N., Abe-Dohmae, S., Takagi, Y.) 4th International Aromatase Conference, Tahoe City, CA, U.S.A., 1996
  9. Aberrant expression of aromatase in breast cancer tissues. (Harada, N.) 4th International Aromatase Conference, Tahoe City, CA, U.S.A., 1996

国内

  1. P450アロマターゼのユニークな発現調節機構-染色体構造との関連- 原田信広、本田 伸一郎、山田和代、堂前純子、吉村憲子、高木康敬 第69回日本生化学会大会・第19回日本分子生物学会年会合同年会 1996




1995年


原著論文

  1. Watanabe, K., Sasano, H., Harada, N., Ozaki, M., Niikura, H., Sato, S., & Yajima, A.
    Aromatase in human endometrial carcinoma and hyperplasia. Immunohistochemical, in situ hybridization, and biochemical studies.
    Am. J. Pathol. 146, 491-500, 1995
    [PubMed]
  2. Koga, N., Nishimura-Kikuchi, N., Hara, T., Harada, N., Ishii, Y., Yamada, H., Oguri, K, & Yoshimura, H.
    Purification and characterization of a new isoform of cytochrome P-450 responsible for 3-hydroxylation of 2, 5, 2', 5'-tetrachlorobiphenyl in hamster liver.
    Arch. Biochem. Biophys. 317, 464-470, 1995
    [PubMed]
  3. Dellovade, T.L., Rissman,E.F., Thompson, N., Harada, N., & Ottinger, M.A.
    Co-localization of aromatase enzyme and estrogen receptor immunoreactivity in the preoptic area during reproductive aging.
    Brain Res. 674, 181-187,1995
    [PubMed]
  4. Harada, N., Utsumi, T., & Takagi, Y.
    Molecular and epidemiological analyses of abnormal expression of aromatase in breast cancer.
    Pharmacogenetics 5, 59-64, 1995
    [PubMed]
  5. Yamada, K., Harada, N., Honda, S., & Takagi, Y.
    Regulation of placenta-specific expresssion of the aromatase cytochrome P-450 gene; Involvement of the trophoblast specific element binding protein.
    J. Biol. Chem. 270, 25064-25069, 1995
    [PubMed]
  6. Foidart, A., Harada, N., &Balthazart, J.
    Aromatase-immunoreactive cells are present in mouse brain areas that are known to express high levels of aromatase activity.
    Cell Tissue Res. 280, 561-574, 1995
    [PubMed]
  7. Foidart, A., Reid, J., Absil, P., Yoshimura, N., Harada, N., & Balthazart, J.
    Critical re-examination of the distribution of aromatase-immunoreactive cells in the quail forebrain using antibodies raised against human placental aromatase and against the recombinant quail, mouse, and human enzyme.
    J. Chemical Neuroanatomy; 8, 267-282, 1995
    [PubMed]
  8. Foidart, A., Telmcani, O., Harada, N., Abe- Dohmae, S., & Balthazart, J.
    Pre- and post-translational regulation of aromatase by steroidal and non- steroidal aromatase inhibitors.
    Brain Res. 701, 267-278, 1995
    [PubMed]

著書

和文(総説その他)

  1. アロマターゼ欠損症 (原田信広)、Molecular Medicine(遺伝子病マニュアル)32, 286-287, 1995
  2. アロマターゼと癌 (原田信広)、Key Word-癌(先端医学社)、20-21, 1995

学会発表

国際

  1. Adrenergic receptor-mediated regulation of aromatase gene expression in diencephalic neurons. (Abe-Dohmae, S., Harada, N., &Takagi, Y.) 9th International Conference on Cytochrome P450: Biochemistry, Biophysics and Molecular Biology, Zurich, Switzerland, 1995
  2. Regulatory mechanisms of tissue-specific expression in human P-450 aromatase gene. (Harada, N., Yoshimura, N., Takagi, Y.) 9th International Conference on Cytochrome P450: Biochemistry, Biophysics and Molecular Biology, Zurich, Switzerland, 1995
  3. Regulationof placenta-specific expression of the aromatase cytochrome P450 gene. (Yamada, K., Honda, S., Harada, N., Takagi, Y.) 9th International Conference on Cytochrome P450: Biochemistry, Biophysics and Molecular Biology, Zurich, Switzerland, 1995




1994年


原著論文

  1. Foidart, A., Clerk A.D., Harada, N., & Balthazarty, J.
    Aromatase-immunoreactive cells in the quail brain: Effects of testosterone and sex dimorphism.
    Physiol. Behav. 55 (3), 453-464, 1994
    [PubMed]
  2. Dellovade, T.L., Harada, N., & Rissman, E.F.
    Distribution and steroid dependence of aromatase enzyme immunoreactivity in limbic nuclei of the female musk shrew brain.
    Brain Res. 634, 141-149, 1994
    [PubMed]
  3. Honda, S., Harada, N., & Takagi, Y.
    Novel exon 1 of the aromatase gene specific for aromatase transcripts in human brain.
    Biochem. Biophys. Res. Commun. 198 (3), 1153-1160, 1994
    [PubMed]
  4. Abe-Dohmae, S., Tanaka, R., & Harada, N.
    Cell type- and region-specific expression of aromatase mRNA in cultured brain cells.
    Mol. Brain Res. 24,153-158, 1994
    [PubMed]
  5. Sasano, H., Nagura, H., Harada, N., Goukon Y., & Kimura, M.
    Immunolocalization of aromatase and other steroidgenic enzymes in human breast disorders.
    Human Pathol. 25, 530-535, 1994
    [PubMed]
  6. Santen, R.J., Martel, J., Hoagland, M., Naftolin, F., Roa, L., Harada, N., Hafer, L., Zaino, R., & Santner, S.J.
    Stromal spindle cells contain aromatase in human breast tumors.
    J. Clin. Endocrinol. Metab. 79, 627-632, 1994
    [PubMed]
  7. Balthazart, J., Stoop, R., Foidart, A., & Harada, N.
    Synergistic control by androgens and estrogens of aromatase in the quail brain.
    NeuroReport 5, 1729-1732, 1994
    [PubMed]
  8. Sigle, R.O., Titus, M.A., Harada, N., & Nelson, S.D.
    Baculovirus mediated high level expression of human placental aromatase.(CYP19A1).
    Biochem. Biophys. Res. Commun. 201, 694-700, 1994
    [PubMed]
  9. Foidart, A., Harada, N., & Balthazart, J.
    Effects of steroidal and non steroidal aromatase inhibitors on sexual behavior and aromatase-immunoreactive cells and fibers in the quail brain.
    Brain Res. 657, 105-123, 1994
    [PubMed]
  10. Aste, N., Panzica, G.C., Aimar, P., Viglietti-Panzica, C., Harada, N., Foidart, A., & Balthazart, J.
    Morphometric studies demonstrate that aromatase-immunoreactive cells are the main target of androgens and estrogens in the quail medial preoptic nucleus.
    Exp. Brain Res. 101, 241-252, 1994
    [PubMed]
  11. Jakab, R.L., Harada, N., & Naftolin, F.
    Aromatase- (estrogen synthetase) immunoreactive neurons in the rat septal area. A light and electron microscopic study.
    Brain Res. 664, 85-93, 1994
    [PubMed]

著書

欧文(分担執筆共著)

  1. Harada, N., Utsumi, T., & Takagi, Y.
    Tissue-specific expression of cytochorme P-450arom gene by alternative use of multiple exons 1 and promoters, and a switching of tissue-specific exons 1.
    Cytochrome P-450 [M.C. Lechner 編] 141-144, John Libbey Eurotext, 1994
  2. Shimozawa, O., Sakaguchi, M., Ogawa, H., Harada, N., Mihara, K., & Omura, T.
    Core glycosylation of cytochrome P-450(arom): evidence for localization of N-terminus of microsomal cytochrome P-450 in the lumen.
    Cytochrome P-450 [M.C. Lechner 編] 315-318, John Libbey Eurotext, 1994
  3. Yamada, K., Kato, S., Harada, N., & Takagi, Y.
    Expression of aromatase cytochrome P450 in choriocarcinoma cells.
    Cytochrome P-450 [M.C. Lechner 編] 651-653, John Libbey Eurotext, 1994

学会発表

国際

  1. Molecular and epidemiological analyses of abnormal expression of aromatase in breast cancer. (Harada, N.) 14th International Symposium on Cancer : Genetic Polymorphism and Cancer Susceptibility, Sapporo, 1994
  2. Tissue-specific expression of human aromatase gene by alternative use of multiple exons 1 and promoters, and a switching of tissue- specific exons 1. (Harada, N., Utsumi, Takagi, Y.) 9th International Congress on Hormonal Steroid, Dallas, TX, U.S.A., 1994
  3. Induction of aromatase mRNA in the diencephalic neurons cultured in vitro.(Abe-Dohmae, S., Harada, N., Tanaka, R., Takagi, Y.) 9th International Congress on Hormonal Steroid, Dallas, TX, U.S.A., 1994
  4. Novel exon 1 of the aromatase gene specific for aromatase transcripts in the brain. (Honda, S., Harada, N., Takagi, Y.) 9th International Congress on Hormonal Steroid, Dallas, TX, U.S.A., 1994
  5. Aromatase mRNA in benign prostatic hypertrophy and prostatic cancer. (Tsugaya, M., Hayashi, Y., Harada, N., Hirao, N., Tozawa, K., Kohri, K.) 23th World Congress Society International Urology, Sydney, Australia,1994
  6. Synergistic control of sexual behavior by androgens estrogens, and aromatase in quail. (Balthazart, J. Stoop, R., Foidart, A., Harada, N.) 21th International Congress on Ornithology, Vienna, Austria, 1994
  7. Aromatase mRNA in benign prostatic and prostate cancer. (Tsugaya, M., Hayashi, Y., Sasaki, S., Tozawa, K., Kohri, K., Harada, N.) Annual Meeting American Urological Association, Las Vegas, NV, U.S.A., 1994
  8. Regulation of estrogen synthetase in osteoblasts: Induction of aromatase activity and its gene expression by retinoic acid. (Chaki, O., Miyaura, C., Harada, N., Takagi, Y., Gorai, I., Minaguchi, H., Suda, T.) Annual Meeting of American Society for Bone and Mineral Research, Kelseyville, CA, U.S.A., 1994
  9. Identification of aromatase-immunoreactive cells in the quail brain by antibodies raised against recombinant quail, mouse, and human aromatases. (Foidart, A., Reid, J., Yoshimura, N., Harada, N., Balthazart, J.) 24th Annual Meeting of Society for Neuroscience, Miami Beach, FL., U.S.A., 1994
  10. Aromatase induction by testosterone in the quail brain is blocked by steroidal but not by non-steroidal inhibitors. (Balthazart, J., Tlemcani, O., Foidart, A., Abe-Dohmae, S., Harada, N.) 24th Annual Meeting of Society for Neuroscience, Miami Beach, FL., U.S.A., 1994

国内
  1. 脳内P-450アロマターゼの生理機能と組織特異的発現調節 原田信広、堂前純子、 Balthazart, J.、本田伸一郎、山田和代、田中亮、高木康敬 第67回日本生化学会大会 1994
  2. エストロゲン合成酵素(アロマターゼ)の発現調節の分子生物学的解析 原田信広 第67回日本内分泌学会学術総会 1994




1993年


原著論文

  1. Abe-Dohmae, S., Harada, N., Yamada, K., & Tanaka, R.
    bcl-2 gene is highly expressed during neurogenesis in the central nervous system.
    Biochem. Biophys. Res. Commun. 191(3), 915-921, 1993
    [PubMed]
  2. Harada, N.
    Genetic analysis of human placental aromatase deficiency.
    J. Steroid Biochem. Molec. Biol. 44 (4-6), 331-340, 1993
    [PubMed]
  3. Jakab, R.L., Horvath, T.L., Leranth, C., Harada, N., & Naftolin, F.
    Aromatase immunoreactivity in the rat brain: gonadectomy-sensitive hypothalamic neurons and an unresponsive "limbic ring" of the lateral septum-bed nucleus-amygdala complex.
    J. Steroid Biochem. Molec. Biol. 44 (4-6), 481-498, 1993
    [PubMed]
  4. Shimozawa, O., Sakaguchi, M., Ogawa, H., Harada, N., Mihara, K., & Omura, T.
    Core-glycosylation of cytochrome P-450(arom); evidence for localization of N-terminus of microsomal cytochrome P-450 in the lumen.
    J. Biol. Chem. 268 (28), 21399-21402, 1993
    [PubMed]
  5. Harada, N., Dohmae, S., Loeffen, R., Foidart, A., & Balthazart, J.
    Synergism between androgen and estrogens in the induction of aromatase and its messenger RNA in the brain.
    Brain Res. 622, 243-256, 1993
    [PubMed]
  6. Suchi, M., Harada, N., Wada, Y., & Takagi, Y.
    Molecular cloning of a cDNA encoding human histidase.
    Biochim. Biophys. Acta 1216, 293-295, 1993
    [PubMed]
  7. Yamada, K., Harada, N., Tamaru, M., & Takagi, Y.
    Effects of changes in gonadal hormones on the amounts of aromatase messenger RNA in mouse brain diencephalon.
    Biochem. Biophys. Res. Commun. 195 (1), 462-468, 1993
    [PubMed]
  8. Harada, N., Utsumi, T., & Takagi, Y.
    Tissue-specific expression of the human aromatase cytochrome P-450 gene by alternative use of multiple exons 1 and promoters, and switching of tissue-specific exons 1 in carcinogenesis.
    Proc. Natl. Acad. Sci. USA. 90 (23), 11312-11316, 1993
    [PubMed]

著書

和文(総説その他)

  1. 脳の性分化および性行動の制御因子としてのエストロゲン合成酵素(P-450・アロ マターゼ):その多様な生理機能と発現調節機構(原田信広)、生化学、65 (2), 67-85, 1993
  2. P-450aromatase欠損症:ステロイドホルモン異常症ー分子生物学的アプローチ、(原田信広)、最新医学、48 (8), 1216-1222, 1993
  3. 多重エクソン1/ プロモーターの選択と組織特異的発現 (原田信広)、細胞工学、12 (12), 880-886, 1993

学会発表

国際

  1. Aromatase-immunoreactive cells are present in the mouse and rat brain areas that express high levels of aromatase activity. (Foidart, A., Absil, N., Harada, N., & Balthazart, J.) 23rd Annual Meeting of Society for Neuroscience, Washington, D.C., U.S.A., 1993
  2. Projective septal neurons are immunoreactive for the estrogen-synthesizing enzyme aromatase. Are there "estrogenergic" neural pathways in the brain? (Jakab, R.L., Harada, N., & Naftolin, F.) 23rd Annual Meeting of Society for Neuroscience, Washington, D.C., U.S.A., 1993
  3. Core-glycosylation of cytochrome P-450arom : Evidence for localization of N-terminus of microsomal cytochrome P-450 in the lumen. (Shimozawa, O., Sakaguchi, M., Ogawa, H., Harada, N., Mihara, K., & Omura, T.) 8th International Conference on Cytochrome P450 : Biochemistry, Biophysics and Molecular Biology, Lisbon, Portugal, 1993
  4. Tissue-specific expression of cytochrome P-450arom gene by alternative use of multiple exons 1 and promoters, and a swithcing of tissue-specific exons 1. (Harada, N., Honda, S., & Takagi, Y.) 8th International Conference on Cytochrome P450 : Biochemistry, Biophysics and Molecular Biology, Lisbon, Portugal, 1993
  5. Expression of aromatase cytochrome P-450 in choriocarcinoma cells. (Yamada, K., Katoh, S., Harada, N., & Takagi, Y.) 8th International Conference on Cytochrome P450 : Biochemistry, Biophysics and Molecular Biology, Lisbon, Portugal, 1993

国内

  1. ヒト・アロマターゼの発現異常と発癌 原田信広 第52回日本癌学会総会 1993




1992年


原著論文

  1. Harada, N., Ogawa, H., Shozu, M., Yamada, K., Suhara, K., Nishida, E., & Takagi, Y.
    Biochemical and molecular genetic analyses on placental aromatase (P-450AROM) deficiency.
    J. Biol. Chem. 267(7), 4781-4785, 1992
    [PubMed]
  2. Balthazart, J., Surlemont, C., & Harada, N.
    Aromatase as a cellular marker of testosterone action in the preoptic area.
    Physiol. Behav. 51, 395-409, 1992
    [PubMed]
  3. Takagi, Y., Ogawa, H., Harada, N., Shimada, H., Ishimura, Y., & Takagi, Y.
    Expression and transport into mitochondoria of bovine cytochrome P-450(SCC) in insect cells using the baculovirus expression system.
    Biochem. Biophys. Res. Commun. 184(1), 471-477, 1992
    [PubMed]
  4. Balthazart, J., Foidart, A., Surlemont, C., Harada, N., & Naftolin, F.
    Neuroanatomical specificity in the autoregulation of aromatase- immunoreactive neurons by androgens and estrogens: an immunocytochemical study.
    Brain Res. 574, 280-290, 1992
    [PubMed]
  5. Harada, N., Yamada, K., Foidart, A., & Balthazart, J.
    Regulation of aromatase cytochrome P-450 (estrogen synthetase) transcripts in the quail brain by testosterone.
    Mol. Brain Res. 15, 19-26, 1992
    [PubMed]
  6. Harada, N., Ogawa, H., Shozu, M., & Yamada, K.
    Genetic studies to characterize the origin of the mutation in placental aromatase deficiency.
    Am. J. Hum. Genet. 51, 666-672, 1992
    [PubMed]
  7. Harada, N. & Yamada, K.
    Ontogeny of aromatase messenger ribonucleic acid in mouse brain: fluorometrical quantitation by polymerase chain reaction.
    Endocrinology 131(5), 2306-2312, 1992
    [PubMed]
  8. Harada, N.
    A unique aromatase (P-450AROM) mRNA formed by alternative use of tissue-specific exons 1 in human skin fibroblasts.
    Biochem. Biophys. Res. Commun. 189(2), 1001-1007, 1992
    [PubMed]

著書

和文(総説その他)

  1. mRNAの微量測定法(原田信広)、細胞工学、11 (10), 771-775, 1992
  2. P-450・アロマターゼ(エストロゲン合成酵素)の構造と機能(原田信広)、蛋白質・核酸・酵素、37 (16), 3051-3059, 1992

学会発表

国際

  1. Genetic analysis of human placental aromatase deificiency. (Harada, N.) Internation Conference on Aromatase, Bologna, Italy, 1992
  2. Two aromatase-immunoreactive neuronal systems in the rat brain. (Jakab, R.L., Naftolin, F., Harada, N., & Leranth, C.) International Conference on Aromatase, Bologna, Italy, 1992
  3. Aromatase-immunoreactive cells in the quail preoptic area: Sex dimorphism and effects of testosterone. (Foidart, A., DeAlerk, A., Harada, N., & Balthazart, J.) 22nd Annual Meeting of Society for Neuroscience, Anaheim, CA, U.S.A., 1992
  4. Synergism between androgen and estrogen in the induction of aromatase in the quail brain: immunocytochemical and RT-PCR studies. (Balthazart, J., Foidart, A., Loeffen, R., Dohmae, S., & Harada, N.) 22nd Annual Meeting of Society for Neuroscience, Anaheim, CA, U.S.A., 1992

国内

  1. アロマターゼの生理機能と発現制御 原田信広 第65回日本生化学会大会 1992




1991年


原著論文

  1. Balthazart, J., Foidart, A., Surlemont, C., & Harada, N.
    Distribution of aromatase-immunoreactive cells in the mouse forebrain
    Cell Tissue Res. 263, 71-79, 1991
    [PubMed]
  2. Balthazart, J., Foidart, A., Surlemont, C., & Harada, N.
    Neuroanatomical specificity in the co- localization of aromatase and estrogen receptors.
    J. Neurobiol. 22(2), 143-157, 1991
    [PubMed]

学会発表

国際

  1. Aging and preoptic area aromatase immunoreactivity in male Japanese quail. (Ottinger, M.A., Thompson, N., Dellovade, T., Harada, N., & Rissman, E.F.) Neuroscience Annual Meeting, New Orleans, LA, U.S.A., 1991
  2. Catecholaminergic innervation of aromatase and estrogen receptor-immunoreactive cells in the quail brain. (Bailhache, T., Foidart, A., Surlemont, C., Harada, N., & Balthazart, J.) Neuroscience Annual Meeting, New Orleans, LA, U.S.A., 1991
  3. Neuroanatomical specificity in the induction of aromatase by androgens and estrogens: An immunocytochemical study. (Balthazart, J., Foidart, A., Surlemont, C., & Harada, N.) Neuriscience Annual Meeting , New Orleans, LA, U.S.A., 1991
  4. Expression of estrogen synthetase (aromatase) by an undifferentiated lung cancer : Report of a novel mechanism of gynecomastia. (Stein, P., True, L., Sullivan, W., Harada, N., & Naftolin, F.) Endocrine Society Annual Meeting, Washington, DC, U.S.A., 1991




1990年


原著論文

  1. Balthazart, J., Foidart, A., Surlemont, C., Vockel, A., & Harada, N.
    Distribution of aromatase in the brain of the Japanese quail, ring dove and zebra finch: and immunocytochemical study.
    J. Comp. Neuol. 301, 276-288, 1990
    [PubMed]
  2. Yamada, K. & Harada, N.
    Expression of estrogen synthetase (P-450 aromatase) during adipose differentiation of 3T3-L1 cells.
    Biochem. Biophys. Res. Commun. 169(2), 531-536, 1990
    [PubMed]
  3. Balthazart, J., Foidart, A., & Harada, N.
    Immunocytochemical localization of aromatase in the brain.
    Brain Res. 514, 327-333, 1990
    [PubMed]
  4. Harada, N., Yamada, K., Saito, K., Kibe, N., Dohmae, S., & Takagi, Y.
    Structural characterization of the human estrogen synthetase (aromatase) gene.
    Biochem. Biophys. Res. Commun. 166(1), 365-372, 1990
    [PubMed]
  5. Naganuma, M., Ohtasni, H., Harada, N., & Nagura, H.
    Immunoelectron microscopic localization of aromatase in human placenta and ovary using microwave fixation.
    J. Histochem. Cytochem. 38(10), 1427-1432, 1990
    [PubMed]

著書

欧文(分担執筆共著)

  1. Preoptic aromatase in quail: behavioral, biochemical and immunochemical studies. Hormones, Brain and Behavior in Vertebrates. (Balthazart, J., Foidart, A., Surlemont, C., & Harada, N.)2. Behavioral activation in males and females-Social interactions and reproductive physiology.; Com. Physiol. Vol. 9. [J. Balthazart Ed.] ; 45-62, Karger, 1990

学会発表

国際

  1. Structural characterization of the human aromatase gene. (Harada, N., Yamada, K., & Takagi, Y.) International Symposium on Microsomes and Drug Oxidations, Stockholm, Sweden, 1990
  2. Expression of cytochrome P-450 aromatase in differentiating 3T3-L1 cells.(Yamada, K., Harada, N., & Takagi, Y.) International Symposium on Microsomes and Drug Oxidations, Stockholm, Sweden, 1990
  3. Immunocytochemical localization of aromatase and estrogen receptors in the brain. (Balthazart, J., Foidart, A., Surlemont, C., Harada, N., Leranth, C., & Naftolin, F.) Neuroscience Annual Meeting, St. Louis, MO, U.S.A., 1990




1989年


著書

欧文(分担執筆共著)

  1. Molecular Cloning of Human UMP Synthetase. (Suchi, M., Harada, N., Yuboi, T., Wada, Y., & Takagi, Y.) Purine and Pyrimidine Metaborism in Man.; 511-518, Adv. Exp. Med. Biol., 1989

和文(総説その他)
  1. 先天性オロット酸尿症の本態に関する分子遺伝学的研究(須知万里子、原田信広、和田義郎、高木康敬)、日本先天代謝異常学会雑誌、5 (2), 23-33, 1989
  2. 分子内分泌学:エストロゲン合成酵素(アロマターゼ)、(原田信広)日本臨床、47 (10), 2287-2292, 1989




1988年


原著論文

  1. Harada, N. & Negishi, M.
    Substrate specificities of cytochrome P-450, C-P-450 16a and P-450 15a, and contribution to steroid hydroxylase activites in mouse liver microsomes.
    Biochem. Pharmacol. 37, 4778-4780, 1988
    [PubMed]
  2. Harada, N.
    Novel properties of human placental aromatase as cytochrome P-450: Purification and characterization of a unique from of aromatase.
    J. Biochem. 103, 106-113, 1988
    [PubMed]
  3. Harada, N.
    Cloning of a complete cDNA encoding human aromatase: Immunochemical identification and sequence analysis.
    Biochem. Biophys. Res. Commun. 156, 725-732, 1988
    [PubMed]

著書

和文(総説その他)

  1. 先天代謝異常症:遺伝学的研究の進歩(原田信広)、現代医学、35 (3), 443-446, 1988




1987年


学会発表

国際

  1. Purification and characterization of a unique form of human placental  aromatase . (Harada, N.)International Symposium on Microsomes and Drug Oxidations, Adelaide, Australia, 1987




1986年


原著論文

  1. Jigami, Y., Harada, N., Umemura, H., Tanaka, H., Ishikawa, K., Nakasato, S., Kita, H., & Sugiura, M.
    Identification of a polymyxin produced by a synbiotic microorganism isolated from the brown planthopper, Nilaparavata lugens.
    Agr. Biol. Chem. 50, 1637-1639, 1986
    [PubMed]
  2. Muraki, M., Jigami, Y., Tanaka, H., Harada, N., Kishimoto, F., Agui, H., Ogino, S., & Nakasato, S.
    Expression of synthetic human lyxozyme gene in Escherichia coli.
    Agr. Biol. Chem. 50, 713-723, 1986
    [PubMed]
  3. Jigami, Y., Muraki, M., Harada, N., & Tanaka, H.
    Expression of synthetic human-lysozyme gene in Saccharomyces cereviciae : Use of a synthetic chicken-lysoztyme signal sequence for secretion and processing.
    Gene 43, 273-279, 1986
    [PubMed]
  4. Taniguchi, T., Yamauchi, K., Yamamoto, Y., Toyoshima, K., Harada, N., Tanaka, H., Takahashi, H., Yamamoto, H., & Fujimoto, S.
    Depression in gene expression for poly-ADP-ribose) synthetase during the interferon-g-induced acticvation process of murine macrophage tumer cells
    Eur. J. Biochem. 171, 571-575, 1986
    [PubMed]

学会発表

国際

  1. cDNA and deduced amino acid sequences of testosterone 16a-hydroxylase (C-P-450 16a) from male 129/J mice. (Wong, G., Kawajiri, K., Kato, Y., Harada, N., & Negishi, M.) FASEB Meeting, Washington, DC., U.S.A., 1986




1985年


原著論文

  1. Miwa, G.T., Harada, N., & Lu, A.Y.H.
    Kinetic isotope effects on cytochrome P-450-catalyzed oxidation reactions. Full expression of the intrinsic isotope effect during the 0-deethylation of 7-ethoxycoumarin by liver microsomes from 3-methylcholanthrene induced hamsters.
    Arch. Biochem. Biophys. 239 (1), 155-162, 1985
    [PubMed]
  2. Harada, N. & Negishi, M.
    Sex-dependent expression of mouse testosterone 16a-hydroxylase (P-450 16a): cDNA cloning and pretranslational regulationl.
    Proc. Natl. Acad. Sci. U.S.A. 82(4), 2024-2028, 1985
    [PubMed]
  3. Devore, K., Harada, N., & Negishi, M.
    Characterization of testosterone 16a-hydroxylase (1-P-450 16a) induced by phenobarbital in mice.
    Biochemistry 24(20) 5632-5637, 1985
    [PubMed]
  4. Burkhart, B.A., Harada, N., & Negishi, M.
    Sexual dimorphism of testosterone 15a-hydroxylase mRNA levels in mouse liver. c-DNA cloning and regulation.
    J. Biol. Chem. 260(28), 15357-15361, 1985
    [PubMed]




1984年


原著論文

  1. Kuwahara, S., Harada, N., Yoshioka, H., Miyata, T., & Omura, T.
    Purification and characterization of four forms of cytochrome P-450 from liver microsomes of phenobarbital-treated and 3-methylcholanthrene-treated rats.
    J. Biochem. 95, 703-714,
    [PubMed]
  2. Lu, A.Y.H., Harada, N., & Miwa, G.T.
    Rate-limiting steps in cytochrome P-450-catalyzed reactions. Studies on isotope effects in the 0-deethylation of 7-ethoxycoumarin.
    Xenobiotica 14, 19-26,
    [PubMed]
  3. Harada, N., Miwa, G.T., Walsh, J.S., & Lu, A.Y.H.
    Kinetic isotope effexcts in cytochrome P-450-catalyzed oxidation reactions. Evidence for the irreversib le formation of an activated oxygen intermediate of cytochrome P-448.
    J. Biol. Chem. 259(5), 3005-3010,
    [PubMed]
  4. Harada, N. & Negishi, M.
    Mouse liver testosterone 15a-hydroxylase (cytochrome P-450 15a). Purification, regioselectivity, stereospecificity, and sex-dependent expression.
    J. Biol. Chem. 259(2), 1265-1271,
    [PubMed]
  5. Harada, N. & Negishi, M.
    Mouse liver testosterone 16a-hydroxylase (cytochrome P-450 16a). Purification, regioselectivity, stereospecificity, and immunochemical characterization.
    J. Biol. Chem. 259 (19), 12285-12290,
    [PubMed]

学会発表

国際

  1. Effect of postnatal exposure of cimetidine on blood testosterone level and hepatic microsomal testosterone hydroxylation activity in the rat. (Lazarte, R.A., Harada, N., & Negishi, M.) Annual Meeting of the Society for Pediatric Research, Miami, FL, U.S.A., 1984
  2. Characterization of sex-dependent cytochrome P-450 s specific for testosterone 16a- and 15a-hydroxylation activities of mouse liver microsomes. (Harada, N. & Negishi, M.) FASEB Meeting , St. Louis, MO, U.S.A., 1984
  3. Primary isotope effects on cytochrome P-450-catalyzed reactions. (Miwa, G.T., Walsh, J.S., Harada, N., & Lu, A.Y.H.) ACS Meeting, Philadelphia, PA, U.S.A., 1984
  4. The full expression of the intrinsic isotope effect during the O-deethylation of 7-ethoxycoumarin(7EC) by 3-methylcholanthrene (3MC)-treated hamster. (Lu, A.Y.H., Harada, N., & Miwa, G.T.) International Symposium on Microsomes and Drug Oxidations, Brighton, UK, 1984
  5. Characterization of sex-dependent testosterone 16a-hydroxylase (P-45016a) and 15a-hydroxylase (P-450 15a) activities from 129/J mouse liver microsomes. (Harada, N. & Negishi, M.) International Symposium on Microsomes and Drug Oxidations, Brighton, UK, 1984




1983年


原著論文

  1. Harada, N. & Omura, T.
    Phenobabital and 3-methylcholanthrene-induced syntheses of two different molecular species of microsomal cytochrome P-450 in rat liver.
    J. Biochem. 93, 1361-1373,
    [PubMed]

著書

欧文(分担執筆共著)

  1. Comparative biochemistry of animal, plant, and microorganism oxidases. (Omura, T., Harada, N., & Yoshioka, H.) IUPAC Pesticide Chemistry, Human Welfare and the Environment. [Miyamoto, J. 編]; 255-262, Pergamon Press, 1983