APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 64(1) 99-105 2004年3月 [査読有り]
Improper protein-folding often results in inclusion-body formation in a protein expression system using Escherichia coli. To express such proteins in the soluble fraction of E. coli cytoplasm, we developed an expression system by fusing the target...
K Morino   H Katsumi   Y Akahori   Y Iba   M Shinohara   Y Ukai   Y Kohara   Y Kurosawa   
JOURNAL OF IMMUNOLOGICAL METHODS 257(1-2) 175-184 2001年11月 [査読有り]
We developed a system by which antibodies, fused to fluorescent proteins with different wavelengths, can be prepared within a month against various antigens. An antibody library composed of a large number of single-chain Fv-CL fragment was constru...