C Miyazaki Y Iba Y Yamada H Takahashi J Sawada Y Kurosawa
PROTEIN ENGINEERING 12(5) 407-415 1999年5月 [査読有り]
The specificity for 11-deoxycortisol (11-DOC) of a monoclonal antibody (mAb), designated SCET, was changed to specificity for cortisol (CS) by site-specific mutagenesis followed by random mutagenesis. The Fab form of SCET was expressed on the surf...
An attempt was made to change the specificity of antibodies (Abs) by introduction of mutations. A monoclonal Ab specific for 17 alpha-hydroxyprogesterone (17-OHP) was used as the starting Ab, On the basis of a model that was generated by a compute...
We prepared three kinds of phagemid vector that permit the simultaneous introduction of highly diverged sequences into six complementarity-determining regions (CDRs) of an antibody (Ab) by the polymerase chain reaction (PCR) with degenerate oligod...
お知らせ・マニュアル
研究推進本部