Particulated structures of microbes are relatively resistant to paraffin embedding. Nuclear/cytoplasmic inclusions in paraffin-embedded H&
E preparation or ethanol-fixed Papanicolaou-stained cytologic specimens can be targeted for EM observatio...
Acta histochemica et cytochemica 48(5) 153-7 2015年10月 [査読有り]
We performed pre-embedding electron microscopic study for visualizing the antigen and genome of severe fever with thrombocytopenia syndrome (SFTS) virus in the cytoplasm of macrophages of the human splenic red pulp, both requesting preheating trea...
Microbiology and immunology 59(1) 13-27 2015年1月 [査読有り]
Streptococcus pyogenes is the main causative pathogen of recurrent tonsillitis. Histologically, lesions of recurrent tonsillitis contain numerous plasma cells. Strep A is an antigenic carbohydrate molecule on the cell wall of S. pyogenes. As expec...
Acta histochemica et cytochemica 48(1) 9-14 2015年 [査読有り]
We established a new "ecological" Grocott stain for demonstrating fungi, based upon a 4R principle of refusal, reduction, reuse, and recycle of waste management. Conventional Grocott stain employs environmentally harsh 5% chromic acid for oxidizat...
BACKGROUND: Loss of adenomatous polyposis coli (APC) gene function results in constitutive activation of the canonical Wnt pathway and represents the main initiating and rate-limiting event in colorectal tumorigenesis. APC is likely to participate...