MALAT-1 noncoding RNA is localized to nuclear speckles despite its mRNA-like characteristics. Here, we report the identification of several key factors that promote the localization of MALAT-1 to nuclear speckles and also provide evidence that MAL...
Akila Mayeda   Adrian R. Krainer   
Alternative pre-mRNA Splicing: Theory and Protocols 320-329 2012年2月 [査読有り]
In vitro splicing assays represent an important technique to analyze, in detail, the basic splicing mechanisms for a pre-mRNA of interest. The cell-free assays, under defined conditions, have many advantages for characterizing the cis-acting eleme...
Molecular and cellular biology 30(9) 2220-8 2010年5月 [査読有り]
Overexpression of high-mobility group A protein 1a (HMGA1a) causes aberrant exon 5 skipping of the Presenilin-2 (PS2) pre-mRNA, which is almost exclusively detected in patients with sporadic Alzheimer's disease. An electrophoretic mobility shift a...
Genes to cells : devoted to molecular & cellular mechanisms 12(10) 1179-91 2007年10月 [査読有り]
Aberrant exon 5 skipping of presenilin-2 (PS2) pre-mRNA produces a deleterious protein isoform PS2V, which is almost exclusively observed in the brains of sporadic Alzheimer's disease patients. PS2V over-expression in vivo enhances susceptibility ...
① がん由来細胞を用いた、mRNA再スプライシングを含む異常スプライシングの分子機構の解析、
② ヒトの新規スプライシング因子として再発見されたSPF45の、抗がん多剤耐性への関与機構の解析、
*本研究シーズに関する産学共同研究の問い合わせは藤田医科大学産学連携推進セン
ター(fuji-san@fujita-hu.ac.jp)まで