HISTOCHEMISTRY AND CELL BIOLOGY 133(2) 229-239 2010年2月 [査読有り]
Soluble proteins and glycogen particles, which are easily lost upon conventional chemical fixation, have been reported to be better preserved in paraffin-embedded sections by 'cryobiopsy' combined with freeze-substitution fixation (FS). ...
Methods in Molecular Biology 657 167-179 2010年 [査読有り]
The final goal of immunohistochemical studies is that all findings examined in animal experiments should reflect the physiologically functional background. Therefore, the preservation of original components in cells and tissues is necessary for de...
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY 57(9) 883-888 2009年9月 [査読有り]
The purpose of this study was to clarify a previously controversial issue concerning glutamate (Glu) immunoreactivity (IR) in the inner segment (IS) of photoreceptors by using in vivo cryotechnique (IVCT) followed by freeze substitution (FS), whic...
CELL AND TISSUE RESEARCH 337(1) 91-102 2009年7月 [査読有り]
Umbrella cells (UCs) of the epithelium of the urinary bladder have the capacity to control bladder volume by regulating exocytosis/endocytosis of their intracellular discoid vesicles (DVs). Dynamin (Dyn) is a GTPase that promotes endocytic process...
MEDICAL MOLECULAR MORPHOLOGY 42(1) 24-31 2009年3月 [査読有り]
Protein kinases (PKs) phosphorylate proteins at active regions for signal transduction. In this study, normal and hypoxic mouse kidneys were prepared using an "in vivo cryotechnique" (IVCT) and examined immunohistochemically with specific antibodi...
White matter abnormalities have been implicated in psychiatric diseases such as major depressive disorder (MDD) ; however, the underlying mechanisms remain poorly understood. The structure and function of the corpus callosum are particularly vulne...