Ultrastructural analyses with electron microscopy have provided indispensable information to understand physiology and pathology of the nervous system. Recent advancement in imaging methodology paved the way for complete reconstruction of the neur...
In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs 87-92 2015年1月 [査読有り]
It has been difficult to clarify the precise localizations of soluble serum proteins in thymic tissues of living animals with conventional immersion or perfusion fixation followed by alcohol dehydration owing to ischemia and anoxia. In this study,...
In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs 135-140 2015年1月 [査読有り]
Protein kinases (PKs) phosphorylate proteins at active regions for signal transduction. In this study, normal and hypoxic mouse kidneys were prepared using an “in vivo cryotechnique” (IVCT) and examined immunohistochemically with specific antibodi...
In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs 33-38 2015年1月 [査読有り]
We performed immunohistochemical or ultrastructural analyses of living mouse small intestines prepared by “in vivo cryotechnique” (IVCT). Living morphological states of small intestinal tissues, including flowing erythrocytes and opening blood ves...
Sei Saitoh   Nobuo Terada   Nobuhiko Ohno   Shinichi Ohno   
In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs 51-56 2015年1月 [査読有り]
To identify immunoglobulin (Ig)-producing cells with immunohistochemistry, conventional methods of preparation using chemical fixatives have problems such as the artificial diffusion of components and antigen masking. The “diffusion artifact” is c...
White matter abnormalities have been implicated in psychiatric diseases such as major depressive disorder (MDD) ; however, the underlying mechanisms remain poorly understood. The structure and function of the corpus callosum are particularly vulne...