Frontiers in Endocrinology 13 941166-941166 2022年7月 [査読有り]
Human stem cell-derived organoid culture enables the in vitro analysis of the cellular function in three-dimensional aggregates mimicking native organs, and also provides a valuable source of specific cell types in the human body. We previously es...
Yu Kodani   Miho Kawata   Hidetaka Suga   Yoko S Kaneko   Akira Nakashima   Toshiki Kameyama   Kanako Saito   Hiroshi Nagasaki   
eNeuro 2022年4月
Hypothalamic melanin-concentrating hormone (MCH) neurons are important regulators of multiple physiological processes, such as sleep, feeding, and memory. Despite the increasing interest in their neuronal functions, the molecular mechanism underly...
The hypothalamus is comprised of heterogenous cell populations and includes highly complex neural circuits that regulate the autonomic nerve system. Its dysfunction therefore results in severe endocrine disorders. Although recent experiments have ...
Journal of neural transmission (Vienna, Austria : 1996) 2020年8月 [査読有り]
5'-Nucleotidase domain-containing protein 2 (NT5DC2) has been revealed by genome-wide association studies (GWAS) as a gene implicated in neuropsychiatric disorders related to the abnormality of dopamine (DA) activity in the brain. Based on its ami...
Nakashima A   Yamaguchi H   Kodani Y   Kaneko YS   Kawata M   Nagasaki H   Nagatsu T   Ota A   
Biochemical and biophysical research communications 516(4) 1060-1065 2019年9月 [査読有り]
A Nakashima   N Hayashi   K Mori   YS Kaneko   T Nagatsu   A Ota   
FEBS LETTERS 465(1) 59-63 2000年1月
Tyrosine hydroxylase (TH), which converts L-tyrosine to L-3,4-dihydroxyphenylalanine, is a rate-limiting enzyme in the biosynthesis of catecholamines; its activity is regulated by the feedback inhibition of the catecholamine products including dop...
M Ota   A Nakashima   K Mori   T Hamanaka   A Ota   
BIOGENIC AMINES 15(2) 197-216 1999年
To determine if the antagonism of serotonin 5HT(2A) receptors and dopamine D-2 receptors by risperidone alters the mRNAs encoding the transporters and enzymes that are involved in re-uptake of serotonin and dopamine and in the degradation and refi...
A Nakashima   K Mori   T Nagatsu   A Ota   
JOURNAL OF NEURAL TRANSMISSION 106(9-10) 819-824 1999年
Wild-type and N-terminal 35-, 38-, and 44-amino acid-deleted mutants of human tyrosine hydroxylase type 1 (hTH1) fused to maltose-binding protein via the target sequence for a restriction protease were expressed in Escherichia coli and purified. T...
K Mori   A Nakashima   T Nagatsu   A Ota   
NEUROSCIENCE LETTERS 238(1-2) 21-24 1997年11月
The amounts of messenger RNA for three enzymes, namely guanosine triphosphate (GTP) cyclohydrolase I, 6-pyruvoyltetrahydropterin synthase, and sepiapterin reductase, all of which are involved in the de novo biosynthesis of (GR)-L-erythro-dihydroxy...