Frontiers in Endocrinology 13 941166-941166 2022年7月 [査読有り]
Human stem cell-derived organoid culture enables the in vitro analysis of the cellular function in three-dimensional aggregates mimicking native organs, and also provides a valuable source of specific cell types in the human body. We previously es...
Yu Kodani   Miho Kawata   Hidetaka Suga   Yoko S Kaneko   Akira Nakashima   Toshiki Kameyama   Kanako Saito   Hiroshi Nagasaki   
eNeuro 2022年4月
Hypothalamic melanin-concentrating hormone (MCH) neurons are important regulators of multiple physiological processes, such as sleep, feeding, and memory. Despite the increasing interest in their neuronal functions, the molecular mechanism underly...
The hypothalamus is comprised of heterogenous cell populations and includes highly complex neural circuits that regulate the autonomic nerve system. Its dysfunction therefore results in severe endocrine disorders. Although recent experiments have ...
Journal of neural transmission (Vienna, Austria : 1996) 2020年8月 [査読有り]
5'-Nucleotidase domain-containing protein 2 (NT5DC2) has been revealed by genome-wide association studies (GWAS) as a gene implicated in neuropsychiatric disorders related to the abnormality of dopamine (DA) activity in the brain. Based on its ami...
Nakashima A   Yamaguchi H   Kodani Y   Kaneko YS   Kawata M   Nagasaki H   Nagatsu T   Ota A   
Biochemical and biophysical research communications 516(4) 1060-1065 2019年9月 [査読有り]
A Ota   A Nakashima   K Mori   T Nagatsu   
NEUROSCIENCE LETTERS 229(1) 57-60 1997年6月
N-Terminus-deleted mutants and wild-type human tyrosine hydroxylase type 1 were expressed in Escherichia coli (E. coli) and utilized to investigate the dopamine-induced decrease in the enzyme catalytic activity and also to identify the specific po...
A Nakashima   K Mori   S Sasaki   
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 228(3) 846-851 1996年11月
We investigated the mechanism by which polyanions gelatinized nuclei of some mouse lymphocytes and ruptured these cells. The gel produced by the addition of dextran sulfate (DS) to mouse lymphocyte nuclei was composed of histones (H1, H2A, H2B, H3...