Biochemical and biophysical research communications 385(2) 170-5 2009年7月 [査読有り]
Vitamin D(3) (VD(3)) is a fat-soluble prohormone that plays a crucial role in bone metabolism, immunity, and control of cell proliferation and cell differentiation in mammals. The actinomycete Pseudonocardia autotrophica is capable of bioconversio...
Bioscience, biotechnology, and biochemistry 73(4) 805-10 2009年4月 [査読有り]
We report here some efficient biotransformations using Escherichia coli strains with disruptions for the AcrAB-TolC efflux pump system. Biotransformations of compactin into pravastatin (6alpha-hydroxy-iso-compactin) were performed using E. coli st...
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 361(4) 876-882 2007年10月 [査読有り]
Cytochrome P450 MoxA (P450moxA) from a rare actinomycete Nonomuraea recticatena belongs to the CYP105 family and exhibits remarkably broad substrate specificity. Here, we demonstrate that P450moxA acts on several luciferin derivatives, which were ...
Bioscience, biotechnology, and biochemistry 71(9) 2184-9 2007年9月 [査読有り]
Tempeh is a traditional Indonesian soybean-fermented food produced by filamentous fungi, Rhizopus sp. and Fusarium sp. We isolated and sequenced the genomic gene and a cDNA clone encoding a novel protease (FP) from Fusarium sp. BLB. The genomic ge...
Bioscience, biotechnology, and biochemistry 70(9) 2299-302 2006年9月 [査読有り]
A gene for cytochrome P450 (moxA) from Nonomuraea recticatena, coexpressed with camAB for pseudomonad redox partners in Escherichia coli, hydroxylated oleanolic acid to produce queretaroic acid. When we used the P450-induced whole-cell as a cataly...