Genes to cells : devoted to molecular & cellular mechanisms 21(9) 1006-14 2016年9月 [査読有り]
Pre-mRNA splicing is widely repressed upon heat shock in eukaryotic cells. However, it has been shown that HSP105 pre-mRNA is alternatively spliced in response to heat stress. Using RNAi screening in HeLa cells, we found that RNA-binding proteins ...
International journal of molecular sciences 17(8) 2016年8月 [査読有り]
The exon junction complex (EJC) that is deposited onto spliced mRNAs upstream of exon-exon junctions plays important roles in multiple post-splicing gene expression events, such as mRNA export, surveillance, localization, and translation. However,...
Madoka Chinen   Misato Morita   Kazuhiro Fukumura   Tokio Tani   
The Journal of biological chemistry 285(8) 5630-8 2010年2月 [査読有り]
prp13-1 is one of the mutants isolated in a screen for defective pre-mRNA splicing at a nonpermissive temperature in fission yeast Schizosaccharomyces pombe. We cloned the prp13(+) gene and found that it encodes U4 small nuclear RNA (snRNA) involv...
In metazoan organisms, alternative splicing is a central mechanism for the regulation of gene expression. However, many questions remain about the underlying molecular mechanisms. Our recent work suggests that U1 snRNP-independent pre-mRNA splicin...
Nucleic acids research 37(6) 1907-14 2009年4月 [査読有り]
U1 snRNP plays a crucial role in the 5' splice site recognition during splicing. Here we report the first example of naturally occurring U1-independent U2-type splicing in humans. The U1 components were not included in the pre-spliceosomal E compl...