Department of Preventive Medical Sciences,
Faculties of Medical Sciences, Fujita Health University

News & Topics

  • 8 Jun 2022
   Our research paper entitled Associations of Genome-Wide Polygenic Risk Score and Risk Factors With Hypertension in a Japanese Population was published in Circulation: Genomic and Precision Medicine. (here
  • 10 Apr 2022
 We have a new member. (Ms.Watanabe, M1) 

  • 21 Dec 2021
 Mr. Yoshiki Tsuboi, Assistant, received the excellent presentation award at The 80th Annual Meeting of Japanese Society of Public Health.
  • 30 Aug 2021
 Mr. Yoshiki Tsuboi, Assistant, received JSPS KAKENHI Grant-in-Aid for Research Activity Start-up (21K21205).
  • 12 Aug 2021
 Our research paper entitled “DNA methylation as a mediator of associations between the environment and chronic diseases: A scoping review on application of mediation analysis” was accepted in Epigenetics. (here) (Press release: here)
  • 22 Jun 2021
 Our research paper entitled “Analysis of Repeated Measurements of Serum Carotenoid Levels and All-Cause and Cause-Specific Mortality in Japan” was featured in Medical Tribune. (here)

Introduction

In the Department of Preventive Medical Sciences, we conduct molecular epidemiological studies on the development of non-communicable diseases. For example, we are constructing risk model for disease onset using genetic information and analyzing biomarkers that mediate between exposure (life-style) and outcome (disease). Our goal is to contribute to preventive medicine by predicting the disease onset or elucidating the molecular mechanism.
If you are interested in Epidemiology, biomarker analysis, prevention of disease onset, and/or statistical analysis, welcome to visit our laboratory and join us.

Members

Faculty members

Medical Technology

Graduate students

Doctor’s course: Yuji Hattori (D3), Yoshiki Tsuboi (D3), Itsuki Kageyama (D2), Yuki Nouchi (D2)
Master’s course: Mami Watanabe (M1)

Research Theme

Molecular Epidemiological study on life-style and life-style related diseases in general population

We participate in health check-ups in Yakumo town, Hokkaido, which has been held since 1982 (Yakumo Study). About 500 residents participate this health check-ups in every year. We conduct molecular epidemiological study on life-styles and onset of life-style related diseases.

Recently, it has been suggested that epigenetics, a mechanism that regulates gene expression regardless of the gene sequence, plays an important role in the development of life-style related diseases. We analyze DNA methylation and miRNA, which is one of the Epigenetics, to elucidate mechanism of development of diseases and establish biomarkers useful for risk assessment.

A large-scale genetic epidemiological study for chronic disease in a Japan population

To realize preventive medicine, it is important to estimate disease risks incorporating genetic factors and lifestyle habits in a Japanese population. Our on-going project constructs a risk estimation model that combines genetic factors and lifestyle habits, focusing on hypertension and renal function. The genetic data about 15,000 Japanese is obtained from the Japan Multi-Institutional Collaborative Cohort (J-MICC) Study, which is one of the largest genome cohort studies in Japan.

Other collaborative research

  • Japan Collaborative Cohort Study (JACC Study) here
  • Research project for prospective Investigation of health problems Among Survivors of the Great East Japan Earthquake and Tsunami Disaster (RIAS Study) here

Annual Events

Health check-ups in the Yakumo, Hokkaido, Japan

Scene of the Health check-up

In every August, we participate in the annual health check-ups in the town of Yakumo, Hokkaido, Japan, where we conduct questionnaires and collect blood and urine samples. This event is essential to conducting Epidemiological study.

Conference Presentation

Scene of receiving poster presentation award in the Japanese Society of Public Health

Faculty members and graduate students present their research at academic conferences in Japan and abroad such as Scientific meetings of the Japan Epidemiology Association and the World Congress of Epidemiology.

Journal Club

Once a week, we have a scientific meeting to critically evaluate recent articles with research colleagues.

Lab trip

Every year, we go out to take pictures of the starry sky, autumn leaves etc. In summer, we go on a camping trip with research colleagues to deepen a friendship.

Photo Gallery

  • Medical examination venue

  • Observation deck in Yakumo ikusei bokujyou, Yakumo town

  • Milkey way seen from Yakumo town

  • Presentation in international conference

  • Progress meeting of graduation research

Academic Activities

Research papers (Last updated: 31 Mar 2022)

2022

  •  Hattori Y, Yamada H, Munetsuna E, Ando Y, Mizuno G, Fujii R, Tsuboi Y, Ichino N, Osakabe K, Sugimoto K, Ishikawa H, Ohashi K, Suzuki K. Increased brain-derived neurotrophic factor in the serum of persons with nonalcoholic fatty liver disease. Endocr J. 2022 Mar 31. doi: 10.1507/endocrj.EJ21-0584.
  • Yamazaki M, Yamada H, Munetsuna E, Maeda K, Ando Y, Mizuno G, Fujii R, Tsuboi Y, Ohashi K, Ishikawa H, Hashimoto S, Hamajima N, Suzuki K. DNA methylation level of the gene encoding thioredoxin-interacting protein in peripheral blood cells is associated with metabolic syndrome in the Japanese general population. Endocr J. 2022 Mar 28;69(3):319-326. doi: 10.1507/endocrj.EJ21-0339.
  • Kato D, Takegami Y, Seki T, Nakashima H, Osawa Y, Suzuki K, Yamada H, Hasegawa Y, Imagama S. DNA methylation is associated with muscle loss in community-dwelling older men -the Yakumo study- : a preliminary experimental study. Nagoya J Med Sci. 2022 Feb;84(1):60-68. doi: 10.18999/nagjms.84.1.60.
  •  Ando Y, Yamada H, Munetsuna E, Yamazaki M, Kageyama I, Teshigawara A, Nouchi Y, Fujii R, Mizuno G, Sadamoto N, Ishikawa H, Suzuki K, Hashimoto S, Ohashi K. Maternal high-fructose corn syrup consumption causes insulin resistance and hyperlipidemia in offspring via DNA methylation of the Pparα promoter region. J Nutr Biochem. 2022 Feb 2;103:108951. doi: 10.1016/j.jnutbio.2022.108951.
  •  Zhao W, Morinaga J, Ukawa S, Endo M, Yamada H, Kawamura T, Wakai K, Tsushita, K, Ando M, Suzuki K, Oike Y, Tamakoshi A. Plasma angiopoietin-like protein 2 levels and mortality risk among younger-old Japanese people: a population-based case-cohort study. J Gerontol A Biol Sci Med Sci. 2022 Jan 17:glac017. doi:10.1093/gerona/glac017.

2021

  • Ryosuke Fujii, Shuntaro Sato, Yoshiki Tsuboi, Andres Cardenas, Koji Suzuki. DNA methylation as a mediator of associations between the environment and chronic diseases: A scoping review on application of causal mediation analysis. Epigenetics. 2021;1-27. doi: 10.1080/15592294.2021.1959736.
  • Ryosuke Fujii, Hiroya Yamada, Yoshiki Tsuboi, Yoshitaka Ando, Eiji Munetsuna, Mirai Yamazaki, Koji Ohashi, Hiroaki Ishikawa, Yuya Ishihara, Shuji Hashimoto, Nobuyuki Hamajima, Koji Suzuki. Association between circulating microRNAs and changes in kidney function: a five-year prospective study among Japanese adults without CKD. Clin Chim Acta. 201;521:97-103.
  • Ryosuke Fujii, Yoshiki Tsuboi, Keisuke Maeda, Yuya Ishihara, Koji Suzuki. Analysis of Repeated Measurements of Serum Carotenoid Levels and All-Cause and Cause-Specific Mortality in Japan. JAMA Network Open. 2021;4:e2113369.
  • Hiroya Yamada, Koji Suzuki, Ryosuke Fujii, Miyuki Kawado, Shuji Hashimoto, Yoshiyuki Watanabe, Hiroyasu Iso, Yoshihisa Fujino, Kenji Wakai, Akiko Tamakoshi, and the JACC Study Group.Circulating miR-21, miR-29a, and miR-126 are associated with premature death risk due to cancer and cardiovascular disease: the JACC Study. Sci Rep. 2021;11:5298.
  • Yoshiki Tsuboi, Hiroya Yamada, Eiji Munetsuna, Ryosuke Fujii, Mirai Yamazaki, Yoshitaka Ando, Genki Mizuno, Hiroaki Ishikawa, Koji Ohashi, Shuji Hashimoto, Nobuyuki Hamajima, Koji Suzuki. Global DNA hypermethylation in peripheral blood mononuclear cells and cardiovascular disease risk: a population-based propensity score-matched cohort study. J Epidemiol Community Health. 2021;75:890-895.
  • Sadayuki Ito, Hiroaki Nakashima, Kei Ando, Kazuyoshi Kobayashi, Masaaki Machino, Taisuke Seki, Shinya Ishizuka, Ryosuke Fujii, Yasuhiko Takegami, Hiroya Yamada, Yoshitaka Ando, Koji Suzuki, Yukiharu Hasegawa, Shiro Imagama. Association between Low Muscle Mass and Inflammatory Cytokines. Biomed Res Int. 2021;2021:5572742.
  • Koji Suzuki, Hiroya Yamada, Ryosuke Fujii, Eiji Munetsuna, Yoshitaka Ando, Koji Ohashi, Hiroaki Ishikawa, Mirai Yamazaki, Keisuke Maeda, Shuji Hashimoto, Nobuyuki Hamajima. Association between circulating vascular-related microRNAs and an increase in blood pressure: A five-year longitudinal population-based study. Journal of Hypertension. 2021;39:84-89.
  • Ryosuke Fujii, Hiroya Yamada, Eiji Munetsuna, Mirai Yamazaki, Genki Mizuno, Yoshitaka Ando, Keisuke Maeda, Yoshiki Tsuboi, Koji Ohashi, Hiroaki Ishikawa, Chiharu Hagiwara, Kenji Wakai, Shuji Hashimoto, Nobuyuki Hamajima, Koji Suzuki. Dietary fish and n-3 polyunsaturated fatty acids are associated with leukocyte ABCA1 DNA methylation levels. Nutrition. 2021;81:110951.

  • Eight other reports

2020

  • Keisuke Maeda, Hiroya Yamada, Eiji Munetsuna, Ryosuke Fujii, Mirai Yamazaki, Yoshitaka Ando, Genki Mizuno, Hiroaki Ishikawa, Koji Ohashi, Yoshiki Tsuboi, Shuji Hashimoto, Nobuyuki Hamajima, Koji Suzuki. Association of smoking habits with TXNIP DNA methylation levels in leukocytes among general Japanese population. PloS One 2020;15:e0235486.
  • Koji Suzuki, Nitin Shivappa, Miyuki Kawado, Hiroya Yamada, Shuji Hashimoto, Kenji Wakai, Hiroyasu Iso, Emiko Okada, Ryosuke Fujii, James R. Hébert, Akiko Tamakoshi. Association between dietary inflammatory index and serum C-reactive protein 1 concentrations in the Japan Collaborative Cohort Study. Nagoya J Med Sci. 2020;82:237–249.
  • Mirai Yamazaki, Eiji Munetsuna, Hiroya Yamada, Yoshitaka Ando, Genki Mizuno, Ryosuke Fujii, Hiroaki Ishikawa, Koji Suzuki, Yohei Shimono, Shuji Hashimoto, Koji Ohashi. Maternal fructose consumption downregulates Lxra expression via miR-206-mediated regulation. J Nutr Biochem. 2020; 82:108386.
  • Ryosuke Fujii, Hiroya Yamada, Eiji Munetsuna, Mirai Yamazaki, Koji Ohashi, Hiroaki Ishikawa, Keisuke Maeda, Chiharu Hagiwara, Yoshitaka Ando, Shuji Hashimoto, Nobuyuki Hamajima, Koji Suzuki. Associations of circulating microRNAs (miR-17, miR-21, and miR-150) and chronic kidney disease in a Japanese population. J Epidemiol. 2020;30:177-182.

Three other reports

2019

  • Ryosuke Fujii, Hiroya Yamada, Mirai Yamazaki, Eiji Munetsuna, Yoshitaka Ando, Koji Ohashi, Hiroaki Ishikawa, Haruki Shimoda, Kiyomi Sakata, Akira Ogawa, Seiichiro Kobayashi, Koji Suzuki. Circulating microRNAs (miR-126, miR-197, and miR-223) are associated with chronic kidney disease among elderly survivors of the Great East Japan Earthquake. BMC Nephrol. 2019;20:474.
  • Ryosuke Fujii, Hiroya Yamada, Eiji Munetsuna, Mirai Yamazaki, Yoshitaka Ando, Genki Mizuno, Yoshiki Tsuboi, Koji Ohashi, Hiroaki Ishikawa, Chiharu Hagiwara, Keisuke Maeda, Shuji Hashimoto, Koji Suzuki. Associations between dietary vitamin intake, ABCA1 gene promoter DNA methylation, and lipid profiles in a Japanese population. Am J Clin Nutr. 2019;110:1213-1219.
  • Ryosuke Fujii, Asahi Hishida, Koji Suzuki, Nahomi Imaeda, Chiho Goto, Nobuyuki Hamajima, Kenji Wakai, Takaaki Kondo. CD36 gene polymorphism (rs1761667) is associated with dietary monounsaturated fatty acid intake and hypertension in a Japanese population. Br J Nutr. 2019;121:1215-1222.
  • Ryosuke Fujii, Hiroya Yamada, Eiji Munetsuna, Mirai Yamazaki, Genki Mizuno, Yoshiki Tsuboi, Koji Ohashi, Hiroaki Ishikawa, Yoshitaka Ando, Chiharu Hagiwara, Keisuke Maeda, Shuji Hashimoto, Nobuyuki Hamajima, Koji Suzuki. Dietary vegetable intake is inversely associated with ATP-binding cassette protein A1 (ABCA1) DNA methylation levels among Japanese women. Nutrition. 2019;65:1-5.
  • Mari Kondo, Hiroya Yamada, Munetsuna Eiji, Mirai Yamazaki, Takeshi Hatta, Akihiko Iwahara, Koji Ohashi, Hiroaki Ishikawa, Yoshiki Tsuboi, Takashi Inoue, Ryosuke Fujii, Koji Suzuki. Associations of serum microRNA-20a, -27a, and -103a with cognitive function in a Japanese population: the Yakumo Study. Arch Gerontol Geriatr. 2019;82:155-160.

11 other reports

Conference (only invited or Awarded)

  • Yoshiki Tsuboi The 80th Annual Meeting of Japanese Society of Public Health (Dec, 2021), Excellent Presentation Award
  • Ryosuke Fujii The 91st Annual Meeting of the Japanese Society for Hygiene (Mar, 2021), Young Excellent Presentation Award
  • Koji Suzuki The 30th Annual Meeting of the Japan Epidemiological Association (Feb, 2020), Epidemiology seminar (Presenter)
  • Keisuke Maeda The 78th Annual Meeting of Japanese Society of Public Health (Oct, 2019), Excellent Poster Award

Books

  • 藤井亮輔,鈴木康司著「超入門! Rでできるビジュアル統計学 学会・論文発表に役立つデータ可視化マニュアル」(金芳堂),2021年10月(LINK

Access

  • Access to Preventive Medical Sciences ⇒ University Building 3