Cell Culture Chamber
Safety Cabinet
| Model (Manufacturer) |
MHE-131AJ (SANYO) |
| Features and Specifications, etc. | [Overview] This device allows for aseptic operations such as cell culture and reagent preparation. [Specifications] - Work area dimensions: Width 1,200 mm x Depth 601 mm x Height 680 mm (stainless steel plate) - Front shutter: Reinforced glass with aluminum frame (auto-balance mechanism, UV protection) - Lighting: Fluorescent lamp (FL-40SD), germicidal lamp (GL-15) - Piping: For gas burner, vacuum, and drain valve - Burner: Ignites only when the fan is operating - Intake filter: Standard HEPA filter - Exhaust filter: Low pressure drop HEPA filter - Cleanliness level: Class 100 [Features] - The work area is made of durable stainless steel. - Wide clean work area with minimal airflow disturbance. - Low noise and low vibration due to the small fan motor. [Usage Examples] - Maintenance and subculturing of cultured cells. - Filtration sterilization of media and reagents. [Note] This device maintains a negative pressure inside the chamber to keep the samples clean, thus greatly reducing cross-contamination between the samples and the operator. For this reason, the device can handle samples with high pathogenicity. |
| Location | Biosafety Level 2 room (Room 305, University Building 1) |
| Remarks | - New users are requested to contact the Cell Function Analysis Laboratory. - Please enter your reservation through the reservation system before using. - Please limit the use (reservation) time to up to 3 hours. |
| Equipment Manager Extension Number |
Tezuka (Cell Function Analysis Laboratory) 2570 |
| Usage Fee | 100 yen/hour |
Equipment Photo
Preparation
CO2 Incubator
| Model (Manufacturer) |
MOC-170AICUV-PJ (PHCbi) |
| Features and Specifications, etc. | [Overview] This device can be used for the cultivation of animal cells. [Specifications] - Operating conditions: 37°C (±0.1°C), 5%CO2 (±0.15%CO2) - Content: 165L - Humidity inside the instrument: 95% (±5%) - Number of trays (shelves): 4 - UV lamp: 1 piece - Heating method: DHA method (heater jacket + air jacket) - Temperature control: PID control method - CO2 concentration control: PID control method (dual IR sensors) - Internal circulation system: Breeze mixing - Temperature and concentration display: LCD (color touch panel) - Alarm function: temperature, gas concentration, door open/close - USB ports [Features] - Stable CO2 concentration control. - Large tray area allows for the installation of many culture vessels. - Automatic control reduces condensation inside the vessel. - Easy management of acquired data parameters (internal temperature, CO2 concentration, door open/close, alarms) . - Decontamination of the vessel with hydrogen peroxide is possible (total decontamination process: approx. 135 min.) . [Usage Examples] - Maintenance of cultured cells. |
| Location | Biosafety Level 2 room (Room 305, University Building 1) |
| Remarks | - New users are requested to contact the Cell Function Analysis Laboratory. - Please enter your reservation through the reservation system before using. - Please limit the use (reservation) time to up to 3 hours. |
| Equipment Manager Extension Number |
Tezuka (Cell Function Analysis Laboratory) 2570 |
| Usage Fee | - |
Equipment Photo
Preparation
Optical Microscopes
| Model (Manufacturer) |
IX70 (OLMPUS) |
| Features and Specifications, etc. | [Overview] This device allows for the observation of animal cells being cultured (maintained) in the same room. [Specifications] - Objective Lens: x4, x10, x20 - Eyepiece: x10 [Features] - The camera and computer (Windows 2000 Professional) provided with the camera can be used to take pictures and acquire data. |
| Location | Biosafety Level 2 room (Room 305, University Building 1) |
| Remarks | - New users are requested to contact the Cell Function Analysis Laboratory. |
| Equipment Manager Extension Number |
Tezuka (Cell Function Analysis Laboratory) 2570 |
| Usage Fee | - |
Equipment Photo
Preparation
Gene Delivery Systems
| Model (Manufacturer) |
AMAXANucleofector (Lonza) |
| Features and Specifications, etc. | [Overview] This device allows electroporation (that is, introducing target genes into cells) by applying electrical pulses to the cells. [Specifications] - Graphic display - IC card slots [Features] - Standard equipment for gene transfer devices. - Easy to operate. - Highly efficient introduction of genes (plasmid DNA, shRNA, miRNA, RNA, etc.) in a single cuvette format (100 μL) . - Gene transfer to primary cultured cells and neurons is possible. - High cell viability and cell function can be maintained. - Optimized kits (sold separately) are available for 160 or more cell types. |
| Location | Biosafety Level 2 Room (Room 305, University Building 1) |
| Remarks | - This equipment is available for loan. - If you wish to use the cellular analysis, please contact the Cell Function Analysis Laboratory. |
| Equipment Manager Extension Number |
Tezuka (Cell Function Analysis Laboratory) 2570 |
| Usage Fee | - |
Equipment Photo
Preparation
Gene Delivery Systems
| Model (Manufacturer) |
GenePulserII (BIO-RAD) |
| Features and Specifications, etc. | [Overview] This device allows electroporation (that is, introducing target genes into cells) by applying electrical pulses to the cells. [Specifications] Gene Pulsar II - Output waveform: Exponential decay pulse - Output power: 50 to 2,500V - Voltage adjustment range: 10V increments (High range), 2V increments (Low range) - Volume: 20 to 80 μL, 40 to 400 μL, 200 to 800 μL Pulse Controller (PC) II - For bacteria and yeast - Set resistance value: 50, 100 to 1,000Ω (in 100Ω increments) Capacitance Extender (CE) II - For animal and plant cells - Capacitor setting value: 25 to 3,275 μF (25 μF increments) [Features] - Usable with microorganisms (E. coli, bacteria), yeast, and animal and plant cells. - Parameters (time constant, voltage, pulse interval, pulse duration) can be changed according to the selected waveform. - Protocols for highly versatile cell types have already been established. |
| Location | Biosafety Level 2 room (Room 305, University Building 1) |
| Remarks | - This equipment is available for loan. - If you wish to use the cellular analysis, please contact the Cell Function Analysis Laboratory. |
| Equipment Manager Extension Number |
Tezuka (Cell Function Analysis Laboratory) 2570 |
| Usage Fee | - |
Equipment Photo
Preparation
Cooling Centrifuge
| Model (Manufacturer) |
himacCF7D2 (HITACH) |
| Features and Specifications, etc. | [Overview] The instrument can centrifuge cell samples under cooling. [Specifications] - Swing rotor compatible type - Rotor and capacity: RT5S4, 15mL (4 tubes/bucket, x4), 50mL (1 tube/bucket, x4) - Maximum speed: 7,000 rpm (30 to 7,000 rpm) - Maximum centrifugal acceleration: 6,570 xg (with RT8A rotor) - Temperature setting range: -9 to 40°C - Time setting range: 1 to 99 minutes, continuous (free) - Mode: Manual mode, memory mode (4 patterns) [Features] - Easy to operate. [Usage Examples] - Cell preparation. - Concentration of samples. |
| Location | Biosafety Level 2 room (Room 305, University Building 1) |
| Remarks | - New users are requested to contact the Cell Function Analysis Laboratory. |
| Equipment Manager Extension Number |
Tezuka (Cell Function Analysis Laboratory) 2570 |
| Usage Fee | - |
Equipment Photo
Preparation
Cooling Centrifuge
| Model (Manufacturer) |
MX301 (TOMY) |
| Features and Specifications, etc. | [Overview] This device can centrifuge trace samples at high speed under cooling. [Specifications] - Angle rotor compatible type - Rotor racks and capacities: AR015-24 (2mL x 24 bottles), AR005-24 (0.5mL x 24 bottles), AR500-04 (15mL x 4 bottles, 50mL x 4 bottles) - Maximum speed: 16,000 rpm (3 to 160 rpm/1 to 232 xg) - Maximum RCF: 21,130 xg - Temperature setting range: -9 to 35°C - Time setting range: 1 to 99 minutes, continuous (free) - Mode: Manual mode, memory mode (3 patterns), flash mode, soft brake [Features] - Easy to operate. [Usage Examples] - Cell preparation. - Concentration of samples. |
| Location | Biosafety Level 2 room (Room 305, University Building 1) |
| Remarks | - New users are requested to contact the Cell Function Analysis Laboratory. |
| Equipment Manager Extension Number |
Tezuka (Cell Function Analysis Laboratory) 2570 |
| Usage Fee | - |
Equipment Photo
Preparation
Large High-speed Cooling Centrifuge
| Model (Manufacturer) |
AvantHP-26XPI (Beckman Coulter) |
| Features and Specifications, etc. | [Overview] This machine is capable of high-speed mass centrifugation under cooling. [Specifications] - Angle rotor / Swing rotor compatible type - Rotor capacity: JLA8.100 (6 x 1,000mL, max. 8,000rpm/15,970 xg), JLA10.500 (6 x 500mL, max. 10,000rpm/18,100 xg), JA-12 (12 x 50mL, max. 12,000rpm/23,200 xg) - Centrifuge tubes and bottles: 12PA tube, 40PA tube, 30PC bottle, 50 mL bottle, 500 mL bottle, 1,000 mL bottle - Maximum speed: 26,000 rpm (100 rpm/xg increments) - Maximum centrifugal force: 15,970 xg (with JLA8.1) - Temperature setting range: -10 to 40°C (in 0.1°C increments) - Time setting range: 1min.~99hour 59min., continuous (free) - Control panel [Features] - Autoclave sterilizable for rotors. - A maximum of 6L of sample can be processed (when JLA8.1 is used). [Usage Examples] - Virus enrichment, bacteria separation, cell separation, diffusion separation, organelle (mitochondria, chloroplast) separation, cell membrane separation. |
| Location | Biosafety Level 2 room (Room 305, University Building 1) |
| Remarks | - New users are requested to contact the Cell Function Analysis Laboratory. |
| Equipment Manager Extension Number |
Tezuka (Cell Function Analysis Laboratory) 2570 |
| Usage Fee | - |
Equipment Photo
Preparation
Autoclave for Sterile Surgical Instruments
| Model (Manufacturer) |
LPS-500 (TOMY) |
| Features and Specifications, etc. | [Overview] This device can sterilize items (such as media and equipment) using high-pressure steam. [Specifications] - Temperature setting range: 105 to 135°C - Maximum operating pressure: 0.281MPa - Internal can capacity: 58L - Safety devices: heating prevention inside the body, heating prevention on the outside of the body, pressure prevention outside the body, detection of temperature sensor breaks, air burning prevention, and lid heat cut-off carburetor. [Features] - Easy to operate. - Due to its large capacity, it can process a large volume of items for sterilization at once. [Usage Examples] - Sterilization of heat-resistant instruments. - Sterilization of culture media and buffer solutions. |
| Location | Biosafety Level 2 room (Room 305, University Building 1) |
| Remarks | - New users are requested to contact the Cell Function Analysis Laboratory. - Sterilization equipment for reagents and instruments used in aseptic manipulation. |
| Equipment Manager Extension Number |
Tezuka (Cell Function Analysis Laboratory) 2570 |
| Usage Fee | 100 yen/time |
Equipment Photo
Preparation
Autoclave for Contaminated Waste
| Model (Manufacturer) |
MLS-2420 (SANYO) |
| Features and Specifications, etc. | [Overview] This device can sterilize items (such as media and equipment) using high-pressure steam. [Specifications] - Temperature setting range: 105 to 121°C - Pressure setting range: 0 to 300 kPa - Time setting range: 1 to 180 minutes - Internal can capacity: 20L - Waste tank capacity: 3L [Features] - Easy to operate. [Usage Examples] - Sterilization of pathogenic samples and the tools used to handle them, generated in Room 305 (BSL2) . |
| Location | Biosafety Level 2 room (Room 305, University Building 1) |
| Remarks | - New users are requested to contact the Cell Function Analysis Laboratory. - This equipment is dedicated to the sterilization of contaminants generated in the Room 305 (BSL2) . No contaminants from outside the room can be brought in. |
| Equipment Manager Extension Number |
Tezuka (Cell Function Analysis Laboratory) 2570 |
| Usage Fee | - |
Equipment Photo
Preparation
