Flow Cytometer
Flow Cytometer Analyzer
| Model (Manufacturer) |
FACSCalibur (BD Bioscience) |
| Features and Specifications, etc. | [Overview] This device enables selective analysis of target cells labeled with fluorescent dyes. [Specifications] - 2 laser: 488nm (3), 635nm (1) *Number of detectors in parentheses - 4-color (4 detectors) - 8 parameters - Maximum processing speed: 10,000 events/sec. - Computer (OS): PowerMac G5 (MacOSX) - Software: CellQuest Pro - Data management: FCS files [Features] - Turn the power ON and you can start measuring in about 10 minutes. - Compared to other models, it is easier to operate. - It is ideal as an introductory device for flow cytometry. [Usage Examples] - Phenotypic analysis of cells stained with fluorescently labeled antibodies or cells artificially expressing fluorescent proteins. - Intracellular signaling analysis using fluorescently labeled phosphorylated antibodies. |
| Location | Central Research Center (Room 317, University Building 1) |
| Remarks | - New users are requested to contact the Cell Function Analysis Laboratory. - Please enter your reservation through the reservation system before using. - Please limit the use (reservation) time to up to 3 hours. |
| Equipment Manager Extension Number |
Tezuka (Cell Function Analysis Laboratory) 2570 |
| Usage Fee | 100 yen/10minute |
Equipment Photo
Flow Cytometer Analyzer
| Model (Manufacturer) |
FACSCalibur (BD Bioscience) |
| Features and Specifications, etc. | [Overview] This device enables selective analysis of target cells labeled with fluorescent dyes. [Specifications] - 1 laser: 488nm (3) *The number in parentheses indicates the number of detectors. - 3-color (3 detectors) - 7 parameters - Maximum processing speed: 10,000 events/sec. - Computer (OS): PowerMac G5 (MacOSX) - Software: CellQuest Pro - Data management: FCS files [Features] - Turn the power ON and you can start measuring in about 10 minutes. - Compared to other models, it is easier to operate. - It is ideal as an introductory device for flow cytometry. [Usage Examples] - Phenotypic analysis of cells stained with fluorescently labeled antibodies or cells artificially expressing fluorescent proteins. - Intracellular signaling analysis using fluorescently labeled phosphorylated antibodies. |
| Location | Bantane Laboratory (4F, West Wing) |
| Remarks | - New users are requested to contact Yasuto Kondo in Pediatrics. |
| Equipment Manager Extension Number |
Yasuto Kondo (Pediatrics, Bantane Hospital) 5739 |
| Usage Fee | 100 yen/10minute |
Equipment Photo
Flow Cytometer Analyzer
| Model (Manufacturer) |
CytoFLEX S (Beckman Coulter) |
| Features and Specifications, etc. | [Overview] This device enables selective analysis of target cells labeled with fluorescent dyes. [Specifications] - 4 lasers: 488nm (2), 638nm (3), 405nm (4), 561nm (4) *The number in parentheses indicates the number of detectors. - 13-color (13 detectors) - 15 parameters - Computer (OS): Windows 10 Professional ver. 20H2 - Software: CytExpert - Data management: FCS files [Features] - Capable of simultaneous fluorescence analysis of up to 13 colors. - Automatic compensation function. - Detectors using Avalanche photodiodes provide excellent red sensitivity. - High resolution (<300nm particles (488nm-SSC), <80nm particles (405nm-SSC). [Usage Examples] - Phenotypic analysis of cells stained with fluorescently labeled antibodies or cells artificially expressing fluorescent proteins. - Intracellular signaling analysis using fluorescently labeled phosphorylated antibodies. |
| Location | Central Research Center (Room 317, University Building 1) |
| Remarks | - New users are requested to contact the Cell Function Analysis Laboratory. - Please enter your reservation through the reservation system before using. - Please limit the use (reservation) time to up to 3 hours. |
| Equipment Manager Extension Number |
Tezuka (Cell Function Analysis Laboratory) 2570 |
| Usage Fee | 100 yen/10minute |
Equipment Photo
Flow Cytometer Analyzer
| Model (Manufacturer) |
Gallios (Beckman Coulter) |
| Features and Specifications, etc. | [Overview] This device enables selective analysis of target cells labeled with fluorescent dyes. [Specifications] - 3 lasers: 488nm (5), 640nm (3), 405nm (2) *The number in parentheses indicates the number of detectors. - 10-color (10 detectors) - 62 parameters - Maximum analysis speed: 25,000 events/sec - Computer (OS): Windows Vista Business - Software: Gallios Software - Data management: LMD files [Features] - Capable of simultaneous fluorescence analysis of up to 10 colors. - Capable of performing cell analysis at high speed. - Automated measurement through integration of the autosampler and software. [Usage Examples] - Phenotypic analysis of cells stained with fluorescently labeled antibodies or cells artificially expressing fluorescent proteins. - Intracellular signaling analysis using fluorescently labeled phosphorylated antibodies. |
| Location | Central Research Center (Room 317, University Building 1) |
| Remarks | - New users are requested to contact the Cell Function Analysis Laboratory. - Please enter your reservation through the reservation system before using. - Please limit the use (reservation) time to up to 3 hours. |
| Equipment Manager Extension Number |
Tezuka (Cell Function Analysis Laboratory) 2570 |
| Usage Fee | 100 yen/10minute |
Equipment Photo
Flow Cytometer Cell Sorter
| Model (Manufacturer) |
MoFlo Astrios High-speed Cell Sorter (Beckman Coulter) |
| Features and Specifications, etc. | [Overview] This device can selectively and with high purity sort (sort) target cells labeled with fluorescent dyes from heterogeneous cell populations. [Specifications] - 2 laser: 488nm (5), 640nm (3) *Number of detectors in parentheses - 8-color (8 detectors) - Maximum analysis speed: 100,000 events/sec - Maximum sorting speed: 70,000 events/sec (Jet-in-Air method) - Measurement particle size range: 0.2 to 30 μm - Sorting: 2-way sorting (15mL, 50mL), 6-way sorting (5mL), plate sorting (96-well) - Touch panel - Computer (OS) : Windows 7 Professional - Software: Summit Software ver. 6.3.1 - Data management: FCS files [Features] - Capable of simultaneous fluorescence analysis of up to 8 colors. - Due to the Jet-in-Air method, high-speed sorting is possible. [Usage Examples] - Capable of sorting cells stained with fluorescently labeled antibodies or cells artificially expressing fluorescent proteins. - Target cells can be sorted as single cells, suitable for gene expression analysis. |
| Location | Central Research Center (Room 317, University Building 1) |
| Remarks | - New users are requested to contact the Cell Function Analysis Laboratory. - The administrator will handle the machine setup and reservation entries prior to use, so those wishing to use it are requested to contact the Cell Function Analysis Laboratory in advance. |
| Equipment Manager Extension Number |
Tezuka (Cell Function Analysis Laboratory) 2570 |
| Usage Fee | 1,000 yen/hour |
Equipment Photo
Flow Cytometer Cell Sorter
| Model (Manufacturer) |
BD FACSAria III Cell Soter (BD Biosceince ) |
| Features and Specifications, etc. | [Overview] This device can selectively sort (sort) target cells labeled with fluorescent dyes from heterogeneous cell populations. [Specifications] - 4 lasers: 488nm (2), 633nm (3), 405nm (2), 561nm (5) *The number in parentheses indicates the number of detectors. - 12-color (12 detectors) - Maximum sorting speed: 30,000 events/sec (cuvette flow cell method) - Measurement particle size range: 0.5 to 30 μm - Sorting: 2-way sorting (15mL), 4-way sorting (2mL, 5mL), plate sorting (96-well) - Computer (OS): Windows 10 - Software: BD FACS Diva softwar ever. 9.0 - Data management: FCS files [Features] - Capable of simultaneous fluorescence analysis of up to 12 colors. - Easier to operate than the MoFlo Astrios. [Usage Examples] - Capable of sorting cells stained with fluorescently labeled antibodies or cells artificially expressing fluorescent proteins. - Target cells can be sorted as single cells, suitable for gene expression analysis. |
| Location | Central Research Center (Room 317, University Building 1) |
| Remarks | - New users are requested to contact the Cell Function Analysis Laboratory. - Those wishing to use it are requested to contact the Cell Function Analysis Laboratory in advance (before reservation entries). - Accudrop Beads must be purchased by users. |
| Equipment Manager Extension Number |
Tezuka (Cell Function Analysis Laboratory) 2570 |
| Usage Fee | 1,000yen/hour |
Equipment Photo
Flow Cytometry Analysis Software
| Model (Manufacturer) |
Kaluza (Beckman Coulter) |
| Features and Specifications, etc. | [Overview] This equipment can thoroughly analyze and graph data obtained through flow cytometry. [Specifications] - Software: Kaluza Soft ver. 2.1 [Features] - Compatible with data obtained from almost all flow cytometers. - Various analyses are possible through drag-and-drop functionality. - Capable of processing list mode files conforming to the FCS standard up to version 3.1. - Multi-color data can be analyzed easily and efficiently through four simple control panels. - Capable of real-time processing of multi-color files with up to 20 million events. - An innovative interface designed with a priority on workflow and user-friendliness. |
| Location | Central Research Center (Room 317, University Building 1) |
| Remarks | - New users are requested to contact the Cell Function Analysis Laboratory. - Please enter your reservation through the reservation system before using. - Please limit the use (reservation) time to up to 3 hours. |
| Equipment Manager Extension Number |
Tezuka (Cell Function Analysis Laboratory) 2570 |
| Usage Fee | - |
Equipment Photo
