Electrophoresis Apparatus
Microchip Electrophoresis Apparatus
| Model (Manufacturer) |
2100 Bioanalyzer (Agilent) |
| Features and Specifications, etc. | Electrophoresis device for DNA, RNA, and proteins (microchip type) 【Features】 - Sensitivity: Can measure concentrations down to pg/μL - Sample volume: DNA/RNA: 1 μL, proteins: 4-5 μL - One device can perform electrophoresis of DNA, RNA, and proteins in about 30 minutes - Using internal standards to automatically correct/calculate data (mobility, molecular size, quantitative values) - Electrode cartridge replacement and cleaning are possible for each assay. 【Electrode Cartridge】 - Dedicated to DNA and RNA (nano) applications. - Dedicated to RNA (pico) applications. * Prior to use, please perform cleaning of the pin electrodes. (Please refer to the electrode cartridge cleaning instructions.) * If you wish to conduct protein electrophoresis, please contact the responsible personnel. 【Specifications】 - Detection method: Laser fluorescence detection - Excitation wavelength, detection wavelength: 630 nm, 680 nm - Optical axis adjustment: Automatically performed for each chip 【Applications】 - Evaluation of RNA sample integrity using RIN (RNA Integrity Number), quality control of DNA and protein samples, etc. |
| Location | Central Research Center (Room 317, University Building 1) |
| Remarks | - There are two types of electrode cartridges available: for DNA and RNA (nano), and RNA (pico) applications. Please use the corresponding cartridge. - Prior to use, please clean the pin electrodes of the electrode cartridge. (Please refer to the electrode cartridge cleaning instructions.) * If you are unsure about the cleaning method, please contact the responsible personnel. - Please ensure to book this equipment through the reservation system when using it. - When using the equipment, please be sure to fill out the logbook provided with it. |
| Equipment Manager Extension Number |
Tsukamoto (Laboratory for Proteins and Genes) 9928 |
| Usage Fee | - |
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Nucleic Acid Electrophoresis Analyzer
| Model (Manufacturer) |
TapeStation 4200 (Agilent) |
| Features and Specifications, etc. | 【Features】 - An electrophoresis system for high-throughput analysis of multiple DNA and RNA samples in a short time, fully automated. - Since Ready-to-use ScreenTape is utilized, there is no need for chip preparation such as gel loading, enabling higher throughput analysis. - Sample loading, electrophoresis, and data analysis are all automated, providing a fully automated analysis process. It's possible to analyze from 1 to 96 samples freely, allowing for efficient use of reagents even when there are few samples, starting from just one. Analysis is feasible with just 1-2 µL per sample, allowing for analysis with minimal sample volume. - It is compatible with 8-tube strips and 96-well plates, making it suitable for quality checking of DNA/RNA samples in high-throughput next-generation sequencing, microarray, qPCR, and transgenic mouse genotyping applications. 【Specifications】 - Up to 96 samples can be analyzed within approximately 90 minutes, with the option to independently switch chips for each sample in separate lanes. - There is a lineup of D1000, D5000, High Sensitivity D1000, High Sensitivity D5000, genomic DNA, RNA, and High Sensitivity RNA tapes, allowing selection based on sample type, concentration, and purpose. |
| Location | Laboratory of Genome Sequencing and Analysis (Room 303 University Building 1) |
| Remarks | When using this equipment, please consult the person in charge. |
| Equipment Manager Extension Number |
Sugihara (Laboratory of Genome Sequencing and Analysis) 9372 |
| Usage Fee | - |
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Fully Automatic Luminescence and Fluorescence Western Blot Device
| Model (Manufacturer) |
Jess (ProteinSimple) |
| Features and Specifications, etc. | Prior to initial use, it is necessary to undergo training.Please contact the following personnel for assistance: An automated device for protein separation via Western blotting and immunoassay detection. 【Features】 Simply load the samples and reagents into the dedicated microplate, and the system will automatically perform size-based protein electrophoresis, antibody addition, incubation, washing, and detection steps, all within approximately 3 hours. 【Specifications】 - Supported functions: size assay (luminescence), size assay (fluorescence), total protein size assay, total protein normalization - Maximum number of samples per measurement: 13 or 25 - Measurement time: Approximately within 3 hours - Control and analysis software: Compass Installed in October 2020 |
| Location | Central Research Center (University Building 1, Room 317) |
| Remarks | - Prior to the initial use, it is necessary to undergo training.Please contact the following personnel for assistance: - Please prepare the separation module kit and detection module (for luminescence or fluorescence) . - Please ensure to book this equipment through the reservation system when using it. - When using the equipment, please be sure to fill out the logbook provided with it. |
| Equipment Manager Extension Number |
Tsukamoto (Laboratory for Proteins and Genes) 9928 |
| Usage Fee | 100 yen/plate |
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