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TOP >  Installed Equipment and Facilities >  PCR equipment

PCR equipment



PCR equipment

Model
(Manufacturer)
PCR Dice Unit 1 (TaKaRa)
Features and Specifications, etc. Device that automatically performs PCR cycles to amplify DNA.

【Features】
- Capable of setting either 96 tubes of 0.2 ml each or one 96-well tube plate.
- Equipped with a gradient function.

【Specifications】
- Heating and cooling method: Peltier element.
- Heating rate: Maximum 3.0 ℃per second.
- Cooling rate: Maximum 2.0 ℃ per second.
- Temperature setting range: 4 to 99.9 ℃ (in 0.1 ℃ increments) .
- Heat lid: 110 ℃
- Gradient function: Adjustable range: 40 to 75 ℃ (temperature span: 6 to 20 ℃).
Location Central Research Center (Room 317, 3rd floor, University Building 1)
Remarks - Please ensure to book this equipment through the reservation system when using it.
- When using the equipment, please be sure to fill out the logbook provided with it.
Equipment Manager
Extension Number
Tsukamoto (Laboratory for Proteins and Genes)
9928
Usage Fee 100 yen/run
Equipment Photo

PCR equipment

Model
(Manufacturer)
PCR Dice Unit 2 (TaKaRa)
Features and Specifications, etc. Device that automatically performs PCR cycles to amplify DNA.

【Features】
- Capable of setting either 96 tubes of 0.2 ml each or one 96-well tube plate.
- Equipped with a gradient function.

【Specifications】
- Heating and cooling method: Peltier element.
- Heating rate: Maximum 3.0 ℃ per second.
- Cooling rate: Maximum 2.0 ℃ per second.
- Temperature setting range: 4 to 99.9 ℃ (in 0.1 ℃ increments) .
- Heat lid: 110 ℃.
- Gradient function: Adjustable range: 40 to 75 ℃ (temperature span: 6 to 20 ℃).
Location Central Research Center (Room 317, 3rd floor, University Building 1)
Remarks - Please ensure to book this equipment through the reservation system when using it.
- When using the equipment, please be sure to fill out the logbook provided with it.
Equipment Manager
Extension Number
Tsukamoto (Laboratory for Proteins and Genes)
9928
Usage Fee 100 yen/run
Equipment Photo

PCR equipment

Model
(Manufacturer)
iCycler (BioRad)
Features and Specifications, etc. Device that automatically performs PCR cycles to amplify DNA.

【Features】
- Capable of setting either 96 tubes of 0.2 ml each or one 96-well tube plate.
- Equipped with a gradient function.

【Specifications】
- Heating and cooling method: Peltier element.
- Heating rate: Maximum 3.3 ℃per second (within the range of 50 to 95 ℃).
- Cooling rate: Maximum 2.0 ℃ per second(within the range of 50 to 95 ℃).
- Temperature setting range: 4 to 100 ℃
- Gradient function: Adjustable range: 40 to 99 ℃ (recommended temperature span: 5 to 25 ℃).
Location Central Research Center (Room 317, 3rd floor, University Building 1)
Remarks - Please ensure to book this equipment through the reservation system when using it.
- When using the equipment, please be sure to fill out the logbook provided with it.
Equipment Manager
Extension Number
Tsukamoto (Laboratory for Proteins and Genes)
9928
Usage Fee 100 yen/run
Equipment Photo

PCR equipment

Model
(Manufacturer)
SimpliAmp Unit 1 (ThermoFisher SCIENTIFIC)
Features and Specifications, etc. Device that automatically performs PCR cycles to amplify DNA.

【Features】
- 96 well, 0.2 ml VeriFlex block.
- VeriFlex block zones: 3 zones.

【Specifications】
- Temperature transition speed: 3.0 to 4.0 ℃ per second.
- Temperature setting range: 0 - 100.0 ℃
- VeriFlex block temperature setting range: maximum 20 ℃ (10 ℃ between adjacent zones), recommended 10 ℃ (5 ℃ between adjacent zones) .
Location Central Research Center (Room 317, 3rd floor, University Building 1)
Remarks - Please ensure to book this equipment through the reservation system when using it.
- When using the equipment, please be sure to fill out the logbook provided with it.
Equipment Manager
Extension Number
Tsukamoto (Laboratory for Proteins and Genes)
9928
Usage Fee 100 yen/run
Equipment Photo

PCR equipment

Model
(Manufacturer)
SimpliAmp Unit 2 (ThermoFisher SCIENTIFIC)
Features and Specifications, etc. Device that automatically performs PCR cycles to amplify DNA.

【Features】
- 96 well, 0.2 ml VeriFlex block.
- VeriFlex block zones: 3 zones.

【Specifications】
- Temperature transition speed: 3.0 to 4.0 ℃ per second.
- Temperature setting range: 0 - 100.0 ℃
- VeriFlex block temperature setting range: maximum 20 ℃ (10 ℃ between adjacent zones), recommended 10 ℃(5 ℃ between adjacent zones) .
Location Central Research Center (Room 317, 3rd floor, University Building 1)
Remarks - Please ensure to book this equipment through the reservation system when using it.
- When using the equipment, please be sure to fill out the logbook provided with it.
Equipment Manager
Extension Number
Tsukamoto (Laboratory for Proteins and Genes)
9928
Usage Fee 100 yen/run
Equipment Photo

Quantitative PCR Equipment

Model
(Manufacturer)
QuantStudio 7 Flex Unit 1 (ThermoFisher SCIENTIFIC)
Features and Specifications, etc. A device for real-time monitoring of DNA amplification by PCR method.

【Features】
- QS7 1 is exclusively for 384-well plates.
- It can accommodate one 384-well plate.

【Specifications】
- 384-well block
- Light source: Halogen lamp
- Excitation at 6 wavelengths (450-670 nm)
 Emission at 6 wavelengths (500-720 nm)

Installed in March 2019.
Location Central Research Center (Room 317, 3rd floor, University Building 1)
Remarks QS7 Unit 1 is exclusively for 384-well plates.
If you are using a 96-well plate, please use QuantStudio 7, unit 2.
- Please ensure to book this equipment through the reservation system when using it.
- When using the equipment, please be sure to fill out the logbook provided with it.
Equipment Manager
Extension Number
Tsukamoto (Laboratory for Proteins and Genes)
9928
Usage Fee 400 yen/run (384-well block)
Equipment Photo

Quantitative PCR Equipment

Model
(Manufacturer)
QuantStudio 7 Flex Unit 2 (ThermoFisher SCIENTIFIC)
Features and Specifications, etc. A device for real-time monitoring of DNA amplification by PCR method.

【Features】
- QS7 Unit 2 is exclusively for 96-well plates.
- It can accommodate one standard 96-well plate (0.2 ml) .
- Fast 96-well plates (0.1 ml) cannot be used.
 
【Specifications】
- 96-well block
- Light source: Halogen lamp
- Excitation at 6 wavelengths (450-670 nm)
 Emission at 6 wavelengths (500-720 nm)

Installed in September 2022.
Location Central Research Center (Room 317, 3rd floor, University Building 1)
Remarks QS7 Unit 2 is exclusively for 96-well plates.
If you are using a 384-well plate, please use QuantStudio 7, unit 1.
- Please ensure to book this equipment through the reservation system when using it.
- When using the equipment, please be sure to fill out the logbook provided with it.
Equipment Manager
Extension Number
Tsukamoto (Laboratory for Proteins and Genes)
9928
Usage Fee 200 yen/run (96-well block)
Equipment Photo

Digital PCR Equipment

Model
(Manufacturer)
Droplet Digital PCR (BioRad)
Features and Specifications, etc. An instrument for ultra-sensitive absolute quantification analysis of nucleic acids

【Droplet Digital PCR System】
- QX200 Automated Droplet Generator (AutoDG)
- QX200 Droplet Reader
- C1000 Touch Thermal Cycler

Installed in March 2019.
Location Central Research Center (Room 317, 3rd floor, University Building 1)
Remarks - In the following cases, please contact the person in charge.
Refilling ddOCR Droplet Reader Oil
 Refilling Automated Droplet Generation Oil for Probes
 Replacing the waste container of the QX200 Droplet Reader
- If you need a DG32 Automated Droplet Generator Cartridge, please consult the person in charge.
- Please ensure to book this equipment through the reservation system when using it.
- When using the equipment, please be sure to fill out the logbook provided with it.
Equipment Manager
Extension Number
Tsukamoto (Laboratory for Proteins and Genes)
9928
Usage Fee 1,750 yen/run (probe)
1,500 yen/run (Eva green)
8 samples/run
Equipment Photo