Mass Spectrometer
LC-MS
| Model (Manufacturer) |
Orbitrap Astral Zoom Mass Spectrometer（Thermo Fisher Scientific） |
| Features and Specifications, etc. | Accelerate discoveries forward with increased flexibility, faster throughput, deeper coverage, higher sensitivty, and accurate and precise quantitation with the Thermo Scientific Orbitrap Astral Zoom mass spectrometer. Designed to achieve the next generation of breakthroughs in proteomics, metabolomics, lipidomics, and biopharma research with faster scan rates, improved duty cycle, sub-cellular sensitivity, and novel acquisition methods. Turn ideas into insights, advancing science exponentially and driving clinical research forward to interventions that improve outcomes. Upgraded instrument architecture leveraging the power of parallelized high-resolution accurate mass acquisition with the Orbitrap and Astral analyzers to enable: Faster throughput Identify over 8,000 proteins from 300 samples per day Deeper coverage Identify over 200,000 unique peptides from a single-shot experiment in under 1 hour Comprehensively characterize small molecule samples with fragmentation of over 90% of compounds in a single injection with data-dependent acquisition Higher sensitivity Identify over 5,500 proteins from a single cell at a throughput of 50 samples per day Accurate and Precise Quantitation Achieve a linear dynamic range of 6 orders of magnitude for metabolites using simultaneous quantitation and discovery (SQUAD) workflows Increase multiplexing with TMT HR mode to support TMTpro 35plex label reagents experiments Increased Flexibility Perform rapid intact, top-down, and middle-down measurements with the Biopharma option, enabling improved pressure regulation, an extended Orbitrap analyzer scan range up to m/z 8,000, and top-down and middle-down MS2 measurements with the Astral analyzer Leverage faster stepped collision energy to increase spectral quality at speed for applications including N-linked glycoproteomics and metabolomics |
| Location | Omics Analysis Laboratory (Room 304, 3rd floor, University Building 1)（） |
| Remarks | Usage conditions apply. - Contact with the person in charge is required. |
| Equipment Manager Extension Number |
Nishioka（Tanaka） 9814 |
| Usage Fee | This equipment is primarily handled through contract measurement services. - Contract measurement service fees DIA/DDA proteomics 20,000 yen/run Sample preparation for LC-MS 2,600 yen/sample |
Equipment Photo
LC-MS
| Model (Manufacturer) |
- EASY-nLC 1000 - Orbitrap Fusion (Thermo Fisher Scientific) |
| Features and Specifications, etc. | For proteomic analysis - Measures the mass-to-charge ratio and intensity of the ionized sample molecules using the mass spectrometer. - Identifies and quantifies proteins using the analysis software Proteome Discoverer. 【Specifications】 - EASY-nLC1000 Mobile Phase A: water with 0.1% formic acid B: acetonitrile with 0.1% formic acid Trap column: Acclaim PepMap 100 Analytical column: EASY-Spray LC Columns - Orbitrap Fusion A hybrid mass spectrometer that combines three analyzers: quadrupole, Orbitrap, and ion trap. It is equipped with both high qualitative capabilities through MSn and excellent quantitative performance via the quadrupole. |
| Location | Central Research Center (Room 317, 3rd floor, University Building 1) |
| Remarks | Usage conditions apply. - Contact with the person in charge is required. |
| Equipment Manager Extension Number |
Nishioka, Tanaka (Omics Analysis Laboratory) 9814 |
| Usage Fee | This equipment is primarily handled through contract measurement services. - Contract measurement service fees 9,900 yen/run for samples of digestion in gel 13,900 yen/run except for samples of digestion in gel |
Equipment Photo
EASY-nLC 1000
Orbitrap Fusion
High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer
(LC-MS/MS)
| Model (Manufacturer) |
LCMS-8060 (SHMADZU) |
| Features and Specifications, etc. | This device analyzes trace components such as drugs and metabolites in the blood with high sensitivity. It is a dedicated instrument for contract analysis, including quantification of primary metabolites, phospholipids, and target compounds. The triple quadrupole mass spectrometer (MS/MS) is a configuration where two quadrupole mass analyzers (Q) are connected in series with a collision cell (q) in between, forming a Q-q-Q setup. Connecting a liquid chromatograph as a pre-processing device forms LC-MS/MS, where the chromatograph serves as the detector. This setup provides high selectivity through mass separation, enabling highly sensitive quantitative analysis. Triple quadrupole mass spectrometers are primarily utilized for quantitative analysis. 【Specifications】 Ion Source Heated ESI (Electrospray Ionization) Probe Sensitivity 1pg Reserpine S/N 1,500,000 Scanning Speed Max 30000 u/sec Mass Range m/z 10-2000 Dwell Time 0.8 ms |
| Location | Omics Analysis Laboratory (University Building 1, Room 304) |
| Remarks | Dedicated Analysis service Machine |
| Equipment Manager Extension Number |
Maeda (Omics Analysis Laboratory) 9814 |
| Usage Fee | - |
Equipment Photo
