Mass Spectrometer
LC-MS
| Model (Manufacturer) |
- EASY-nLC 1000 - Orbitrap Fusion (Thermo Fisher Scientific) |
| Features and Specifications, etc. | For proteomic analysis - Measures the mass-to-charge ratio and intensity of the ionized sample molecules using the mass spectrometer. - Identifies and quantifies proteins using the analysis software Proteome Discoverer. 【Specifications】 - EASY-nLC1000 Mobile Phase A: water with 0.1% formic acid B: acetonitrile with 0.1% formic acid Trap column: Acclaim PepMap 100 Analytical column: EASY-Spray LC Columns - Orbitrap Fusion A hybrid mass spectrometer that combines three analyzers: quadrupole, Orbitrap, and ion trap. It is equipped with both high qualitative capabilities through MSn and excellent quantitative performance via the quadrupole. |
| Location | Omics Analysis Laboratory (Room 304, 3rd floor, University Building 1) |
| Remarks | Usage conditions apply. - Contact with the person in charge is required. |
| Equipment Manager Extension Number |
Nishioka, Tanaka (Omics Analysis Laboratory) 9814 |
| Usage Fee | This equipment is primarily handled through contract measurement services. - Contract measurement service fees 9,900 yen/run for samples of digestion in gel 13,900 yen/run except for samples of digestion in gel |
Equipment Photo
EASY-nLC 1000
Orbitrap Fusion
LC-MS
| Model (Manufacturer) |
Q Exactive (ThermoFisher SCIENTIFIC) (Thermo Fisher Scientific) |
| Features and Specifications, etc. | Quadrupole/Orbitrap Hybrid Mass Spectrometer This equipment is used for proteome analysis, which involves fragmenting proteins and measuring their mass, then comparing this data to the predicted protein amino acid sequences from genomic information to comprehensively analyze the proteins in the sample. This device is capable of identifying trace amounts of proteins and detecting post-translational modifications such as phosphorylation. (Usage examples) Comprehensive analysis of total proteins extracted from cells and tissues, identification of band proteins from SDS-PAGE, comprehensive analysis of co-precipitates from immunoprecipitation, identification of protein phosphorylation, etc. Excellent quantification capabilities. - Advanced Quadrupole Technology (AQT) improves the selectivity and transmission of precursor ions, enhancing the accuracy and sensitivity of quantifying trace samples in complex matrices. - Sophisticated Data Independent Analysis (DIA) and Parallel Reaction Monitoring (PRM) achieve reliable and reproducible quantification while capturing qualitative information. Analysis results with high accuracy and reliability. - HR/AM full scan ensures that all sample data is always captured, allowing for retrospective data analysis without the need for reanalysis. - The capability of up to 140,000 FWHM in maximum resolution allows for mass separation even among closely related molecular weights, improving analytical accuracy in sample analyses involving complex matrices. - Full scan mode and All Ion Fragmentation (AIF) mode provide sub-ppm mass accuracy, enabling reliable identification of compounds. - 12Hz high-speed scanning and spectrum multiplexing enable compatibility with high-resolution chromatograms in UHPLC. - During analysis, rapid polarity switching is feasible, maximizing the information obtained in a single analysis. - A variety of fragmentation techniques such as AIF, in-source CID, and HCD support compound identification across a wide range of applications. - Expanding to m/z 6,000 enhances detection capabilities from small molecules to biomacromolecules, improving detection across a wide range of compounds. - With a spectral dynamic range exceeding four digits and femtogram-level sensitivity, simultaneous detection of trace and high-concentration compounds is achievable in a single scan. (HPLC) HTC-PAL Autosampler nano-Advance UHPLC MonoCap C18 Nano-flow (0.1x150mm) column |
| Location | Central Research Center (Room 317, 3rd floor, University Building 1) |
| Remarks | Terms of Use Yes |
| Equipment Manager Extension Number |
Nishioka (Omics Analysis Laboratory) 9814 |
| Usage Fee | - |
Equipment Photo
High Performance Liquid Chromatograph Triple Quadrupole Mass Spectrometer
(LC-MS/MS)
| Model (Manufacturer) |
LCMS-8060 (SHMADZU) |
| Features and Specifications, etc. | This device analyzes trace components such as drugs and metabolites in the blood with high sensitivity. It is a dedicated instrument for contract analysis, including quantification of primary metabolites, phospholipids, and target compounds. The triple quadrupole mass spectrometer (MS/MS) is a configuration where two quadrupole mass analyzers (Q) are connected in series with a collision cell (q) in between, forming a Q-q-Q setup. Connecting a liquid chromatograph as a pre-processing device forms LC-MS/MS, where the chromatograph serves as the detector. This setup provides high selectivity through mass separation, enabling highly sensitive quantitative analysis. Triple quadrupole mass spectrometers are primarily utilized for quantitative analysis. 【Specifications】 Ion Source Heated ESI (Electrospray Ionization) Probe Sensitivity 1pg Reserpine S/N 1,500,000 Scanning Speed Max 30000 u/sec Mass Range m/z 10-2000 Dwell Time 0.8 ms |
| Location | Omics Analysis Laboratory (University Building 1, Room 304) |
| Remarks | Dedicated Analysis service Machine |
| Equipment Manager Extension Number |
Maeda (Omics Analysis Laboratory) 9814 |
| Usage Fee | - |
Equipment Photo
