Metabolic Analyzer
Extracellular Flux Analyzer
| Model (Manufacturer) |
XFe24 (Agilent Technologies) |
| Features and Specifications, etc. | [Features] - It is an analyzer that automatically analyzes cellular metabolism, allowing non-invasive and highly sensitive temporal measurements of major energy metabolism pathways such as glycolysis and aerobic respiration by mitochondria. It finds applications in various research fields including cancer, immunology, diabetes, obesity, neurodegenerative diseases, and cardiovascular diseases. - Simultaneous measurement of two cellular energy metabolisms: mitochondrial respiration ≒ oxygen consumption rate (OCR) and glycolysis ≒ extracellular acidification rate (ECAR) - Detecting the changes in oxygen concentration (pmol/min) and hydrogen ion concentration (mpH/min) in cultured cells or isolated mitochondria using luminescence methods, and calculate OCR and ECAR . - High-throughput and rapid automated measurements: Measurements are performed automatically by culturing cells on dedicated 24-well (XFe24 model) simply setting them into the main unit. [Specifications] - Analytical method: Photoluminescence method - Simultaneous measurement parameters: O2 ・H+ - Number of simultaneous measurement samples: 24 - Number of injection ports: 4 - Injection port volume: 75 µL - Optimal measurement range for OCR (pmol/min) : 50 to 400 - Optimal measurement range for ECAR (mpH/min) : 20 to 120 [URL] https://www.primetech.co.jp/products/tabid/90/pdid/36/language/ja-JP/Default.aspx |
| Location | Central Research Center (Room 317, University Building 1) |
| Remarks | - This equipment can be used after attending a training session conducted by the staff. Please contact the person in charge regarding the training. - When using this device, please be sure to reserve it through the equipment reservation system. - Please be sure to fill in the usage log provided on the equipment whenever you use it. |
| Equipment Manager Extension Number |
Sugihara (Laboratory of Genome Sequencing and Analysis) 9372 |
| Usage Fee | 500 yen/time |
Equipment Photo
